Calretinin and Other CaBPs in the Nervous System

  • John Rogers
  • Masood Khan
  • Jon Ellis
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 269)


At least three CaBPs are abundant in various types of nerve cells : calbindin-D28, calretinin, and parvalbumin. The sequence of chick calretinin, from cDNA clones, is 60% homologous to that of chick calbindin. The genomic calretinin gene has also been partially sequenced. Calretinin is a protein of 29–30 kilodaltons. Antisera have been raised against ß-galactosidase-calretinin fusion proteins, and used to compare the distribution of calretinin with that of calbindin by two-colour immunofluorescence. Some sections have also been stained for parvalbumin. In chick brain and retina, the three proteins are largely in different neurons. Calbindin and calretinin are particularly abundant in some sensory nuclei, and co-expression is more common in peripheral sensory neurons. In rat brain, and in retinae of rat, cat, and salamander, some of the expression patterns are conserved, but some are not. In the chick embryonic retina, some cells show a transient phase of calbindin immunoreactivity during development.


Olfactory Bulb Amacrine Cell Horizontal Cell Intron Position Chick Brain 
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  1. Baimbridge, K.G., and Miller, J.J., 1982, Immunohistochemical localization of calcium-binding protein in the cerebellum, hippocampal formation and olfactory bulb of the rat. Brain Res., 245: 223–229.PubMedCrossRefGoogle Scholar
  2. Braun, K., Schachner, M., Scheich, H., and Heizmann, C.W., 1986, Cellular localization of the Cabp paralbumin in the developing avian cerebellum. Cell Tissue Res., 243:69–78.CrossRefGoogle Scholar
  3. Celio, M.R., and Heizmann, C.W., 1981, Cabp parvalbumin as a neuronal marker. Nature. 293:300–302.PubMedCrossRefGoogle Scholar
  4. Feldman, S.C., and Christakos, S., 1983, Vitamin D-dependent calcium-binding protein in rat brain: biochemical and immunocytochemical characterization. Endocrinology, 112: 290–302.PubMedCrossRefGoogle Scholar
  5. Garcia-Segura, L.M., Baetens, D., Roth, J., Norman, A.W. and Orci, L., 1984, Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system, Brain Res., 296:75–86.PubMedCrossRefGoogle Scholar
  6. Heizmann, C.W., and Celio, M.R., 1987, Immunolocalization of parvalbumin, Methods in Enzvmology. 139:552–570.CrossRefGoogle Scholar
  7. Jande, S.S., Maler, L., and Lawson, D.E.M., 1981, Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain, Nature. 294:765–767.PubMedCrossRefGoogle Scholar
  8. Jande, S.S., Tolnai, S., and Lawson, D.E.M., 1981, Immunohistochemical localization of vitamin D-dependent Cabp in duodenum, kidney, uterus and cerebellum of chickens, Histochemistry. 71:99–116.PubMedCrossRefGoogle Scholar
  9. Manalan, A.S., and Klee, C.B., 1984, Purification and characterization of a novel Cabp (CBP-18) from bovine brain. J. Biol. Chem., 259:2047–2050.PubMedGoogle Scholar
  10. Matute, C., and Streit, P., 1986, Monoclonal antibodies demonstrating GABA-like immunoreactivity. Histochemistry. 86:147–157.PubMedCrossRefGoogle Scholar
  11. Parmentier, M., Ghysens, M., Rypens, F., Lawson, D.E.M., Pasteels, J.L.,and Pochet, R., 1987, Calbindin in vertebrate classes : immunohistochemical localization and Western blot analysis, General and Comparative Endocrinology, 65:399–407.PubMedCrossRefGoogle Scholar
  12. Pochet, R., Parmentier, M., Lawson, D.E.M. &Pasteels, J.L., 1985, Rat brain synthesizes two ’vitamin D-dependent’ calcium-binding proteins. Brain Research. 345:251–256.PubMedCrossRefGoogle Scholar
  13. Rogers, J.H., 1987, Calretinin : a gene for a novel calcium-binding protein expressed principally in neurons, Journal of Cell Biology. 105:1343–1353.PubMedCrossRefGoogle Scholar
  14. Rogers, J.H., 1989a, Two Cabps mark many chick sensory neurons, Neuroscience. in press.Google Scholar
  15. Rogers, J.H., 1989b, Immunoreactivity for calretinin and other Cabps in cerebellum, Neurosciences. in press.Google Scholar
  16. Roth, J., Baetens, D., Norman, A.W., and Garcia-Segura, L.-M., 1981, Specific neurons in chick central nervous system stain with an antibody against chick intestinal vitamin D-dependent calcium-binding protein. Brain Res., 222: 452–457.PubMedCrossRefGoogle Scholar
  17. White, J.G., Amos, W.B., and Fordham, M., 1987, An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. Journal of Cell Biology. 105:41–48.PubMedCrossRefGoogle Scholar
  18. Wilson, P.W., Harding, M., and Lawson, D.E.M., 1985, Putative amino acid sequence of chick calcium-binding protein deduced from a complementary DNA sequence. Nucl. Acids Res., 13: 8867–8881.PubMedCrossRefGoogle Scholar
  19. Wilson, P.W., Rogers, J., Harding, M., Pohl, V., Pattyn, G., and Lawson, D.E.M., 1988, Structure of chick chromosomal genes for calbindin and calretinin. J. Mol. Biol., 200: 615–625.PubMedCrossRefGoogle Scholar
  20. Yamakuni, R., Kuwano, R., Odani, S., Kiki, N., Yamaguchi, Y., and Takahashi, Y., 1986, Nucleotide sequence of cDNA to mRNA for a cerebellar Cabp, spot 35 protein. Nucl. Acids Res., 14:6768.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • John Rogers
    • 1
  • Masood Khan
    • 1
  • Jon Ellis
    • 1
  1. 1.Physiological LaboratoryUniversity of CambridgeCambridgeUK

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