Structural Details of the Interaction of Calmodulin with the Plasma Membrane Ca2+-ATPase
The plasma membrane Ca2+-ATPase is one of the enzymes which are regulated by calmodulin (for a recent review see Carafoli et al., 1988). The interaction between the two proteins has been studied in de tail. The CaM-binding domain of the ATPase has recently been identified (James et al., 1988, Verma et al., 1988), and it has been shown that the Ca2+-ATPase can be fully stimulated by the C-terminal half of CaM (i.e. AA 78–148) but not by the N-terminal half (1-77; see Guerini et al., 1984). Furthermore, it could be demonstrated that the 3rd Ca2+-binding loop of CaM (counted from the N-terminus) is essential for the activation of the ATPase (Guerini et al., 1984). Recently, chemically modified calmodulins provided evidence that arginine and methionine residues located in the C-terminal half of CaM are important for the interaction between CaM and the plasma membrane Ca2+-ATPase (Guerini et al., 1987).
KeywordsErythrocyte Membrane Bovine Brain Amino Acid Replacement Random Coil Conformation Calmodulin Gene
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- Carafoli, E., Krebs, J., and Chiesi, M., 1988, Calmodulin in the transport of calcium across biomembranes, in: “Molecular aspects of cellular regulation”, P. Cohen and C.B. Klee, eds., Vol. V, Elsevier, Amsterdam.Google Scholar
- Schaefer, W.H., Lukas, T.J., Blair, IA., Schultz, J.E., Watterson, D.M., 1987, Amino acid sequence of a novel calmodulin from Paramecium tetraurelia that contains dimethyllysine in the first domain, J. Biol. Chem., 262:1025.Google Scholar
- Verma, A.K., Filoteo, AG., Standord, D.R., Wieben, E.D., Penniston, J.T., Strehler, E.E., Fischer, R., Heim, R., Vogel, G., Mathews, S., Strehler-Page, M.-A., James, P., Vorherr, T., Krebs, J., and Carafoli, E., 1988, Complete primary structure of a human plasma membrane Ca2+ pump, J. Biol. Chem., 263: 14152.PubMedGoogle Scholar
- Vorherr, T., James, P., Krebs, J., Enyedi, A., McCormick, D.J., Penniston, J.T., and Carafoli, E., 1989, The interaction of calmodulin with the calmodulin binding domain of the plasma membrane Ca2+ -pump. Biochemistry, in press.Google Scholar