Phosphorylation of the Calcium-Binding Protein, p68, in the Submembranous Cytoskeleton of Human Placental Syncytiotrophoblast

  • Paul Kenton
  • Peter M. Johnson
  • Paul D. Webb
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 269)


Human placental syncytiotrophoblast expresses cell surface receptors for a number of growth factors including those for epidermal growth factor (EGF) (Richards, 1983), platelet-derived growth factor (PDGF) (Goustin, 1985) and insulin (Lai, 1981). mRNA transcripts coding for the receptor for macrophage colony stimulating factor (M-CSF), have been identified in fetal syncytiotrophoblast (Hoshina, 1985). Cell-surface receptors for transferrin (Loh, 1980) and for the Fc region of human immunoglobulin G (IgG) (Johnson, 1981) are also expressed.


Membrane Vesicle Macrophage Colony Stimulate Factor Pertussis Toxin Epidermal Growth Factor Treatment Phosphoamino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ashendel, C.L., 1985, The phorbol ester receptor: a phospholipid-regulated protein kinase, Biochim. Biophys. Acta, 822:219–242.PubMedGoogle Scholar
  2. van Belle, H., 1972, Kinetics and inhibition of alkaline phosphatases from canine tissues. Biochim. Biophys. Acta. 289:158–168.PubMedGoogle Scholar
  3. Crompton, M.R., Owens, R.J., Totty, N.F., Moss, S.E., Waterfield, M.D., and Crumpton, M.J., 1988, Primary structure of the human, membrane-associated Ca2+ -binding protein p68: a novel member of a protein family, EMBO J., 7:21–27.PubMedGoogle Scholar
  4. De, B.K., Misono, K.S., Lukas, T.J., Mroczkowski, B., and Cohen, S., 1986, A calcium-dependent 35-kilodalton substrate for epidermal growth factor receptor/kinase isolated from normal tissue, J. Biol. Chem., 261:13784–13792.PubMedGoogle Scholar
  5. Goustin, A.S., Betsholtz, C., Pfeifer-Ohlsson, S., Persson, H., Rydnert, J., Bywater, M., Holmgren, G., Hel-din, C.-H., Westermark, B., and Ohlsson, R., 1985, Coexpression of the sis and myc proto-oncogenes in developing human placenta suggests autocrine growth control of trophoblast growth, Cell. 41:301–312.PubMedCrossRefGoogle Scholar
  6. Hoshina, M., Nishio, A., Bo, M., Boime, I., and Mochizuka, M., 1985, The Expression of oncogene fms in human chorionic tissue, Acta Obst. Gynec. Jpn., 12:2791–2798.Google Scholar
  7. Huang, K-S., Wallner, B.P., Mattaliano, R.J., Tizard, R., Burne, C., Frey, A., Hession, C., McGray, P., Sinclair, L.K., Chow, E.P., Browning, J.L., Ramachandran, K.L., Tang, J., Smart, J.E., and Pepinsky, R.B., 1986, Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinase, Cell. 46:191–199.PubMedCrossRefGoogle Scholar
  8. Johnson, P.M., and Brown, P.J., 1981, Fc receptor in the human placenta, Placenta. 2:355–370.PubMedCrossRefGoogle Scholar
  9. Kenton, P., Johnson, P.M., and Webb, P.D., 1989. Biochim. Biophys. Acta, in press.Google Scholar
  10. Khanna, N.C., Tokuda, M., and Waisman, D.M., 1987, Purification of three forms of lipocortin from bovine lung. Cell Calcium. 8:217–228.PubMedCrossRefGoogle Scholar
  11. Lai, W.H., Guyda, H.J., Branchaud, C.L., and Goodyer, C.G., 1985, Insulin-induced receptor regulation in early gestation and term human placental cell cultures. Placenta. 6:505–517.PubMedCrossRefGoogle Scholar
  12. Loh, T.T., Higuchi, DA., van Bockxmeer, F.M., Smith, C.M., and Brown, E.B., 1980, Transferrin receptor on the human placental microvillous membrane. J. Clin. Invest., 65:1182–1191.PubMedCrossRefGoogle Scholar
  13. Nishizuka, Y., 1984, The role of protein kinase C in cell surface signal transduction and tumour promotion, Nature. 308:693–698.PubMedCrossRefGoogle Scholar
  14. Owens, R.J., and Crumpton, M.J., 1984, Isolation and characterization of a novel 68000-M Ca2+ -binding protein of lymphocyte plasma membrane. Biochem. J., 219:309–316.PubMedGoogle Scholar
  15. Owens, R.J., Gallagher, C.J., and Crumpton, M.J., 1984, Cellular distribution of p68, a new calcium-binding protein from lymphocytes. EMBO J., 3:945–952.PubMedGoogle Scholar
  16. Pepinsky, R.B., and Sinclair, L.K., 1986, Epidermal growth factor-dependent phosphorylation of lipocortin, Nature. 321:81–84.PubMedCrossRefGoogle Scholar
  17. Radke, K., Gilmore, T., and Martin, G.S., 1980, Transformation by Rous sarcoma virus: a cellular substrate for transformation-specific protein phosphorylation contains phosphotyrosine. Cell. 21:821–828.PubMedCrossRefGoogle Scholar
  18. Richards, R.C., Beardmore, J.M., Brown, P.J., Molloy, C.M., and Johnson, P.J., 1983, Epidermal Growth Factor receptor on isolated human placental syncytiotrophoblast plasma membrane, Placenta. 4:133–138.PubMedCrossRefGoogle Scholar
  19. Stewart, A.A, Ingebritsen, T.S., and Cohen, P., 1983, The protein phosphatases involved in cellular regulation, Eur. J. Biochem., 132:289–295.PubMedCrossRefGoogle Scholar
  20. Vaughan, M., and Moss, J., 1986, Guanyl nucleotide-binding proteins and regulation of cAMP metabolism, in: “Mechanisms of Insulin Action,” Belfrage, P., Donner, J., and Stralfors, P., eds., Elsevier, Amsterdam.Google Scholar
  21. Webb, P.D., and Mahadevan, L.C., 1987,Calcium-dependent binding proteins associated with human placental syncytiotrophoblast microvillous cytoskeleton. Biochim. Biophys. Acta. 916:288–297.PubMedCrossRefGoogle Scholar
  22. Webb, P.D., and Todd, J., 1988, Plasma membrane attachment of human placental-type alkaline phosphatase to phosphatidylinositol. Eur. J. Biochem., 172:647–652.PubMedCrossRefGoogle Scholar
  23. Yarden, Y., and Ullrich, A., 1988, Molecular analysis of signal transduction by growth factors. Biochemistry. 27:3313–3119.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Paul Kenton
    • 1
  • Peter M. Johnson
    • 1
  • Paul D. Webb
    • 1
  1. 1.Dept. of ImmunologyUniversity of LiverpoolLiverpoolUK

Personalised recommendations