Purification of Complexes between Peptide Antigens and Class II Major Histocompatibility Complex Antigens Using Biotinylated Peptides
The Major Histocompatability Antigens (MHC) are cell surface glycoproteins which are involved in the regulation of the immune response to foreign antigens. The MHC proteins bind peptides generated by cellular processing of native proteins (Babbitt et al., 1985; Buus et al., 1986; Watts & McConnell, 1986; Chen & Parham, 1989), and the peptide-MHC complexes are presented on cell surfaces to T lymphocytes (Schwartz, 1985). Class I MHC proteins maintain surveillance over proteins which are found in the cell cytoplasm, such as newly synthesized viral proteins in an infected cell (Morrison et al., 1987; Moore et al., 1988). The foreign peptide-MHC complexes are recognized by cytotoxic T lymphocytes, which respond by lysing the infected cell.
KeywordsPeptide Binding Histocompatibility Antigen Antigen Binding Site Major Histocompatibility Complex Antigen Free Biotin
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