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Purification of Complexes between Peptide Antigens and Class II Major Histocompatibility Complex Antigens Using Biotinylated Peptides

  • Theodore Jardetzky
  • Joan Gorga
  • Robert Busch
  • Jonathan Rothbard
  • Jack L. Strominger
  • Don Wiley
Part of the NATO ASI Series book series (NSSA, volume 183)

Abstract

The Major Histocompatability Antigens (MHC) are cell surface glycoproteins which are involved in the regulation of the immune response to foreign antigens. The MHC proteins bind peptides generated by cellular processing of native proteins (Babbitt et al., 1985; Buus et al., 1986; Watts & McConnell, 1986; Chen & Parham, 1989), and the peptide-MHC complexes are presented on cell surfaces to T lymphocytes (Schwartz, 1985). Class I MHC proteins maintain surveillance over proteins which are found in the cell cytoplasm, such as newly synthesized viral proteins in an infected cell (Morrison et al., 1987; Moore et al., 1988). The foreign peptide-MHC complexes are recognized by cytotoxic T lymphocytes, which respond by lysing the infected cell.

Keywords

Peptide Binding Histocompatibility Antigen Antigen Binding Site Major Histocompatibility Complex Antigen Free Biotin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Theodore Jardetzky
    • 1
    • 2
  • Joan Gorga
    • 1
  • Robert Busch
    • 3
  • Jonathan Rothbard
    • 1
  • Jack L. Strominger
    • 1
    • 2
  • Don Wiley
    • 1
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyHarvard UniversityCambridgeUSA
  2. 2.Howard Hughes Medical InstituteLondonUK
  3. 3.Laboratory of Molecular ImmunologyImperial Cancer Research InstituteLondonUK

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