Comparison of the Secondary Structures of Human Class I and Class II MHC Antigens by Ftir and CD Spectroscopy
Considerable evidence exists that the structures of class I and class II histocompatibility antigens are similar. Most of the evidence (summarized in Kappes and Strominger, 1988 and Brown et al., 1988) is based on sequence homologies and similarities in domain structure at both the protein and DNA levels. In addition, some T cells that are specific for either class I or class II molecules use the same receptor (Rupp et al, 1985; Marrack and Kappler, 1986). However, the secondary structures of purified class I and class II antigens have not been directly compared.
KeywordsSecondary Structure Circular Dichroism Protein Secondary Structure Histocompatibility Antigen Circular Dichroism Band
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- Alvarez, J., Lee, D.C., Baldwin, S.A. and Chapman, D., 1987, Fourier transform infrared spectroscopic study of the structure and conformational changes of the human erythrocyte glucose transporter, J. Biol. Chem. 262:3502.Google Scholar
- Bolotina, I.A., Chekhov, V.O., Lugauskas, V.Yu. and Ptitsyn, O.B., 1981, Determination of the secondary structure of proteins from the circular dichroism spectra. II. Consideration of the contribution of ß-bends, Mol. Biol. (English Translation of Molekul. Biol.) 14:709.Google Scholar
- Green, N.M. and Melamed, M.D., 1966, Optical rotary dispersion, circular dichroism, and far-ultraviolet spectra of avidin and streptavidin, Biochem. J. 100:614.Google Scholar
- Gorga, J.C., Horejsí, V., Johnson, D.J., Raghupathy, R. and Strominger, J.L., 1987, Purification and characterization of class II histocompatibility antigens from a homozygous human B cell line, J. Biol. Chem. 262:16087.Google Scholar
- Parham, P., Alpert, B.N., Orr, H.T. and Strominger, J.L., 1977, Carbohydrate moiety of HLA antigens. Antigenic properties and amino acid sequences around the site of glycosylation, J. Biol. Chem. 252:7555.Google Scholar
- Sears, D.W. and Beychok, S., 1973, Circular dichroism, in :“Physical Principles and Techniques of Protein Chemistry,” Part C., F.J. Leach, ed., Academic Press, New York, 445.Google Scholar
- Turner, M.J., Cresswell, P., Parham, P., Strominger, J.L., Mann, D.L. and Sanderson, A.R., 1975, Purification of papain-solubilized histocompatibility antigens from a cultured human lymphoblastoid line, RPMI4265, J. Biol. Chem. 250:4512.Google Scholar
- Woody, R.W., 1987, Contributions of tryptophan side-chains to the far-ultraviolet circular dichroism of proteins, Proc. 2nd Intl. Conf. Circular Dichroism, Budapest, 38.Google Scholar