Cloning, Sequencing and Expression of a New β-Galactosidase from the Extreme Thermophilic Sulfolobus Solfataricus
The number of isolated microorganisms surviving and growing in extreme environmental conditions (60–110°C) is rapidly increasing (Stetter, 1986). Comparison of the biology of these thermophilic microorganisms and their successful strategy of living at such high temperatures requires an understanding of both their metabolism and the relationship between the structure and function of their cellular components. In particular, interest in the enzymes from these organisms is growing because their peculiar properties render them a considerable biotechnological potential and industrial tool. In fact, whereas conventional enzymes are irreversibly inactivated by heat, these enzymes, in addition to their thermophilic and thermostable characteristics, show an enhanced activity, in the presence of the common protein denaturants and organic solvents as well as proteolytic enzymes.
KeywordsThermophilic Microorganism Methanosarcina Barkeri Extreme Thermophile Galactosidase Gene Methanococcus Voltae
Unable to display preview. Download preview PDF.
- Cubellis, M.V., Rozzo, C., Montecucchi, P. and Rossi, M. (manuscript in preparation).Google Scholar
- Maniatis, T., Fritsch, E.F. and Sambrook, J., 1982, Molecular Cloning, in: “A Laboratory Manual,” Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
- Pisani, F.M., Relia, R., Rozzo, C., Raia, C.A., Nucci, R., Gambacorta, A., De Rosa, M. and Rossi, M., 1989, Thermostable β-galactosidase from the archaebacterium Sofolobus solfataricus. Purification and properties, Eur. J. Biochem. (in press).Google Scholar
- Reiter, W.D., Palm, P. and Zillig, W., 1988, Transcription termination in the archaebacterium Sulfolobus: signal structure and linkage to transcription initiation, Nucl. Acids Res. 16: 2245.Google Scholar
- Steuer, K.O., 1986, in: “Thermophiles: general molecular and applied microbiology,” T.D. Brock and J.K. Zeikus, eds., Wiley.Google Scholar
- Ulrich, J.T., McFeters, G.A. and Temple, K.L., 1972, Induction and characterization of ß-galactosidase in an extreme thermophile, J. Bacteriol. 110: 691.Google Scholar
- Wich, G., Leinfelder, W. and Boeck, A., 1987, Genes for stable RNA in the extreme thermophile Thermoproteus tenax: introns and transcription signals, EMBO J. 6: 523.Google Scholar
- Woese, C.R. and Wolfe, R.S., 1985, in: “The Bacteria,” C.R. Woese and R.S. Wolfe, eds., 8:561, Academic Press, New York.Google Scholar