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Methods of Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution

  • Brian J. Stockman
  • John L. Markley
Part of the NATO ASI Series book series (NSSA, volume 183)

Abstract

The scope of this review, which is abbreviated from Stockman and Markley (1989) will be limited to 2H-, 13C-, and 15N-assisted NMR spectroscopy of proteins in solution. Since hydrogen, carbon, and nitrogen are ubiquitous in proteins, the procedures presented here are applicable to any protein. Elegant reviews covering NMR spectroscopy of less predominant atoms in proteins have appeared recently: 19F (Ho et al., 1985), 31P (Gorenstein et al., 1989), 113Cd (Summers, 1988). Techniques utilizing stable-isotope-assisted solid state NMR spectroscopy will not be discussed here.

Keywords

Nuclear Magnetic Resonance Nuclear Magnetic Resonance Spectroscopy Amide Proton Nuclear Magnetic Resonance Study Sequential Assignment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Brian J. Stockman
    • 1
  • John L. Markley
    • 1
  1. 1.Department of Biochemistry, College of Agricultural and Life SciencesUniversity of Wisconsin-MadisonMadisonUSA

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