Genetic Analysis of Polypeptide Chain Folding and Misfolding in Vivo
Some 30 years have passed since the first protein structures were determined by X-ray crystallography, and since Anfinsen and co-workers established that the information for determining conformation could be contained solely within the polypeptide chain (see Anfinsen, 1973). The three-dimensional structure of more than 200 proteins is known to atomic resolution. For each of these proteins, and for thousands more, the amino acid sequence is also known exactly. Yet we are only beginning to be able to predict and understand how the sequence of the amino acids along polypeptide chains determines their three-dimensional conformation.
KeywordsPolypeptide Chain Folding Pathway Folding Intermediate Subunit Assembly Chain Association
Unable to display preview. Download preview PDF.
- Creighton, T. E., 1978, Experimental studies of protein folding and unfolding, Prog. Biophys. Mol. Biol. 22: 231–298.Google Scholar
- Fane, B. A., 1988, Genetic analysis of the folding and maturation defects in the P22 tailspike endorhamnosidase, Ph.D. thesis, Massachusetts Institute of Technology.Google Scholar
- Goldenberg, D., 1981, Genetic and biochemical analysis of the folding and subunit assembly of the bacteriophage P22 tail spike protein, Ph.D. thesis, Massachusetts Institute of Technology.Google Scholar
- King, J., 1986, Genetic analysis of protein folding pathways, Biol technology 4: 297–303.Google Scholar
- King, J., Yu, M-H., Siddiqui, J., and Haase, C. A., 1986, Genetic identification of amino acid sequences influencing protein folding pathways, in: Protein Engineering, Applications in Science, Medicine and Industry ( M. Inoue and R. Sarma, eds.) Academic Press, New York, pp. 275–291.Google Scholar
- Sargent, D., Benevides, J. M., Yu, M-H., King, J., and Thomas, Jr., G. J., 1988, Secondary structure and thermostability of the phage P22 tailspike: Analysis of Raman spectroscopy of the wild type protein and a temperature-sensitive folding mutant, J. Mol. Biol. 199: 491–502.PubMedCrossRefGoogle Scholar