Mass Spectrometric Methods for Determination of the Structure of Peptides and Proteins
Mass spectrometry (MS) has long been known as a sensitive method of characterizing compounds that are small, thermally stable, and sufficiently volatile to be vaporized into the vacuum system of the spectrometer. It was not, however, until the early 1980s that the method became applicable to large, polar molecules, with the development by Barber et al. (1981) of fast-atom-bombardment (FAB) ionization. This ionization method produces protonated molecules, (M + H)+, by bombarding a solution of the compound of interest (M)+, in a suitable liquid of very low vapor pressure, such as glycerol, with neutral atoms (or ions) of kilovolt kinetic energy.
KeywordsLeucine Renin Threonine Asparagine Isoleucine
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