Calcium Release in Skinned Muscle Fibers: Effect of Inositol 1,4,5-Trisphosphate

  • Enrique Jaimovich
  • Cecilia Rojas
  • Eduardo Rojas
Part of the Series of the Centro de Estudios Científicos de Santiago book series (SCEC)


A key issue when postulating a role for inositol 1,4,5-trisphosphate (IP3) as a messenger in excitation-contraction coupling in skeletal muscle is whether the effect of exogenous IP3 on skeletal muscle fibers is compatible with the effect postulated for a physiological intracellular transmitter. It is important to establish then whether IP3 acts at concentrations that are low enough, whether its action is fast enough, and whether it is specific and compatible with the known physiological properties of the process.


Sarcoplasmic Reticulum Fiber Bundle Calcium Release Inositol Phosphate Skeletal Muscle Fiber 
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  1. 1.
    Vergara, J., Tsien, R. Y., and Delay, M., 1985, Inositol 1,4,5-trisphosphate: A possible chemical link in excitation-contraction coupling in muscle, Proc. Natl. Acad. Sci. USA 82: 6352–6356.PubMedCrossRefGoogle Scholar
  2. 2.
    Vergara, J., Asotra, K., and Delay, M., 1987, in: Cell Calcium and the Control of Membrane Transport (L. J. Mandel and D. G. Eaton, Eds.), pp. 133–151, The Rockefeller University Press, New York.Google Scholar
  3. 3.
    Volpe, P., Salviati, G., Di Virgilio, F., and Pozzan, T., 1985, Inositol 1,4,5-trisphosphate induces calcium release from sarcoplasmic reticulum skeletal muscle, Nature 316: 347–349.PubMedCrossRefGoogle Scholar
  4. 4.
    Volpe, P., Di Virgilio, F., Pozzan, T., and Salviati, G., 1986, Role of inositol 1,4,5-trisphosphate in excitation-contraction coupling in skeletal muscle, FEBS Lett. 197: 1–4.PubMedCrossRefGoogle Scholar
  5. 5.
    Nosek, T. M., Williams, M. F., Zeigler, J. T., and Godt, R. E., 1986, Inositol trisphosphate enhances calcium release in skinned cardiac and skeletal muscle, Am. J. Physiol. 250: C807–C810.PubMedGoogle Scholar
  6. 6.
    Donaldson, S. K., Goldberg, N. D., Walseth, T. F., and Huettemann, D. A., 1987, Inositol trisphosphate stimulates calcium release from peeled skeletal muscle fibers, Biochem. Biophys. Acta 927: 92–99.PubMedCrossRefGoogle Scholar
  7. 7.
    Rojas, E., Nassar-Gentina, V., Luxoro, M., Pollard, M. E., and Carrasco, M. A., 1987, Inositol 1,4,5-trisphosphate-induced Ca +2 release from sarcoplasmic reticulum and contraction in crustacean muscles, Can. J. Physiol. Pharmacol. 65: 672–680.PubMedCrossRefGoogle Scholar
  8. 8.
    Sherer, N. M., and Ferguson, J. E., 1985, Inositol 1,4,5-trisphosphate is not effective in releasing calcium from skeletal sarcoplasmic reticulum microsomes, Biochem. Biophys. Res. Commun. 128: 1064–1070.CrossRefGoogle Scholar
  9. 9.
    Palade, P., 1987, Drug-induced Ca +2 release from isolated sarcoplasmic reticulum. III. Block of Ca +2-induced Ca+2 release by organic polyamines, J. Biol. Chem. 262: 6149–6154.PubMedGoogle Scholar
  10. 10.
    Mikos, G. J., and Snow, T. R., 1987, Failure of inositol 1,4,5-trisphosphate to elicit or potentiate Ca +2 release from isolated skeletal muscle sarcoplasmic reticulum, Biochim. Biophys. Acta 927: 256–260.PubMedCrossRefGoogle Scholar
  11. 11.
    Lea, T. J., Griffiths, D. J., Treagar, R. T., and Ashley, C. C., 1986, An examination of the ability of inositol 1,4,5-trisphosphate to induce calcium release and tension development in skinned skeletal muscle fibers of frog and crustacea, FEBS Lett. 203: 153–161.CrossRefGoogle Scholar
  12. 12.
    Blinks, J. R., Cai, Y. D., and Lee, N. K. M., 1987, Inositol 1,4,5-trisphosphate causes calcium release in frog skeletal muscle only when transverse tubules have been interrupted, J. Physiol. (London) 394: 39P.Google Scholar
  13. 13.
    Walker, J. W., Somlyo, A. V., Goldman, Y. E., Somlyo, A. P., and Trentham, D. R., 1987, Kinetics of smooth and skeletal muscle activation by laser pulse photolysis of caged inositol 1,4,5-trisphosphate, Nature 327: 249–252.PubMedCrossRefGoogle Scholar
  14. 14.
    Rojas, C., Rojas, E., Kukuljan, M., and Jaimovich, E., 1988, Inositol 1,4,5-trisphosphate induces calcium release from skinned frog skeletal muscle, Biophys. J. 53: 467a.Google Scholar
  15. 15.
    Rojas, C., and Jaimovich, E., 1990, Calcium release modulated by inositol trisphosphate in skinned fibers from frog skeletal muscle, Eur. J. Physiol, (in press).Google Scholar
  16. 16.
    Donaldson, S. K., 1988, Transverse tubule voltage control of inositol trisphosphate-induced Ca2+ release in peeled skinned muscle fibers, Biophys. J. 53: 468a.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Enrique Jaimovich
    • 1
  • Cecilia Rojas
    • 1
  • Eduardo Rojas
    • 2
  1. 1.Departamento de Fisiología y Biofísica, Facultad de MedicinaUniversidad de ChileSantiagoChile
  2. 2.Laboratory of Cell Biology and Genetics, National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaUSA

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