Monoclonal Antibodies as Probes of Triad Structure and Excitation-Contraction Coupling in Skeletal Muscle

  • Mario S. Rosemblatt
  • Gonzalo Pérez
  • Bojena Antoniu
  • Evelyn Reilley
  • Noriaki Ikemoto
Part of the Series of the Centro de Estudios Científicos de Santiago book series (SCEC)


It is generally accepted that the transverse tubules (T-tubules), a membrane system produced by invaginations of the plasma membrane in skeletal muscle, play a central role in the process of coupling the depolarization of the plasma membrane and muscle contraction (excitation-contraction coupling). However, the detailed molecular mechanism by which the T-tubules communicate with the intracellular membrane system of the sarcoplasmic reticulum (SR) where calcium is stored is largely unknown. Ultrastructural studies of skeletal muscle show that the T-tubules are connected to the SR at both sides through specific structures (the “feet”), forming a structural and functional unit known as a triad(1)


Sarcoplasmic Reticulum None None French Press Frog Skeletal Muscle Transverse Tubule 
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  1. 1.
    Franzini-Armstrong, C., 1977, Structure of sarcoplasmic reticulum, Fed. Proc. 39: 2403–2409.Google Scholar
  2. 2.
    Franzini-Armstrong, C., and Ninzi, G., 1983, Junctional feet and particles in the triad of the fast-twitch muscle fiber, J. Muse. Res. Motility 4: 233–252.CrossRefGoogle Scholar
  3. 3.
    Rosemblatt, M., Nelson, R., and Ikemoto, N., 1984, Inhibition of T-tubule/SR linkage by anti-T-tubule monoclonal antibodies, Biophys. J. 455: 398a.Google Scholar
  4. 4.
    Lau, H. Y., Caswell, A. H., and Brunschwig, J. P., 1977, Isolation of transverse tubules by fractionation of triad junctions of skeletal muscle, J. Biol. Chem. 252: 5565–5574.PubMedGoogle Scholar
  5. 5.
    Perez, G., and Rosemblatt, M., 1986, Immunological studies on surface membranes and transverse tubules isolated from frog skeletal muscle, Arch. Biol. Medicina Experiment. Chile 19: R239.Google Scholar
  6. 6.
    Hidalgo, C., Parra, C., Riquelme, G., and Jaimovich, E., 1986, Transverse tubules from frog skeletal usele: Purification and properties of vesicles sealed with inside-out orientation, Biochem. Biophys. Acta 855: 79–88.PubMedCrossRefGoogle Scholar
  7. 7.
    Beeler, T. J., Gable, K. S., and Effer, J. M., 1983, Characterization of the membrane bound Mg2+ -ATPase of rat muscle. Biochem. Biophys. Acta 734: 221–234.PubMedCrossRefGoogle Scholar
  8. 8.
    Moulton, M. P., Sabbadini, R. A., Norton, K. C., and Dahms, A. S., 1986, Studies on the transverse tubule Mg2+-ATPase: Lectin-induced alteration of kinetic behavior, J. Biol. Chem. 262: 12244–12251.Google Scholar
  9. 9.
    Damiani, E., Margreth, A., Furlan, A., Dahms, A. S., Arnn, J., and Sabbadini, R. A., 1987, Common structural domains in the sarcoplasmic reticulum Ca2+ -ATPase and the transverse tubule Mg2+ -ATPase, J. Cell Biol. 104: 46111–472.CrossRefGoogle Scholar
  10. 10.
    Hidalgo, C., Gonzalez, E., and Lagos, R., 1983, Characterization of the Ca2+ — or Mg2+-ATPase of transverse tubule membrane from rabbit skeletal muscle, J. Biol. Chem. 258: 13937–13945.PubMedGoogle Scholar
  11. 11.
    Tada, M., Yamamoto, T., and Tonomura, Y., 1978, Molecular mechanism of active calcium transport by sarcoplasmic reticulum, Physiol. Rev. 58: 1–79.PubMedGoogle Scholar
  12. 12.
    Rosemblatt, M., Cifuentes, M. E., and Ikemoto, N., 1988, Immunological studies of the Ca2+ release component of sarcoplasmic reticulum, Biophys. J. 61: 32a.Google Scholar
  13. 13.
    Lai, F. A., Erickson, H. P., Rousseau, E., Liu, Q-Y., and Meissner, G., 1988, Purification and reconstitution of the calcium release channel from skeletal muscle, Nature 331: 315–318.PubMedCrossRefGoogle Scholar
  14. 14.
    Imagawa, T., Smith, J. S., Coronado, R., and Campbell K., 1987, Purified ryonodine receptor from skeletal muscle sarcoplasmic reticulum is the Ca-permeable pore of the calcium release channel, J. Biol. Chem. 262: 16636–16643.PubMedGoogle Scholar
  15. 15.
    Ikemoto, N., Antoniu, B., and Kim, D. H., 1984, Rapid calcium release from the isolated sarcoplasmic reticulum is triggered via the attached transverse tubular system, J. Biol. Chem. 259: 13151–13158.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Mario S. Rosemblatt
    • 1
    • 2
  • Gonzalo Pérez
    • 1
  • Bojena Antoniu
    • 2
  • Evelyn Reilley
    • 2
  • Noriaki Ikemoto
    • 2
    • 3
  1. 1.Unidad de Immunología Celular, Instituto de Nutrición y Tecnología de los AlimentosUniversidad de ChileSantiagoChile
  2. 2.Department of Muscle ResearchBoston Biomedical Research InstituteBostonUSA
  3. 3.Department of NeurologyHarvard Medical SchoolBostonUSA

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