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Proteins of the Triad Junction of Skeletal Muscle

  • Anthony H. Caswell
  • Neil R. Brandt
  • Shu-Rong Wen
  • Jane A. Talvenheimo
Part of the Series of the Centro de Estudios Científicos de Santiago book series (SCEC)

Abstract

The basic mechanism of excitation-contraction coupling in skeletal muscle is still not understood despite many years of active research on both the physiology and the biochemistry of the process. Several years ago we embarked on a project of identifying the constituents of the triad junction with the view that this junctional region represents not only the point of physical contact between the external and internal organelle but also the point of dynamic transmission in muscle excitation.(1,2) In 1986 we described the isolation of a protein of approximate subunit molecular weight 300,000 which spanned the gap between the transverse (T) tubule and the terminal cisternae, and therefore represented a portion of the junctional feet(3) Later work by others has demonstrated that this protein is identical to the ryanodine receptor protein and contains Ca2+ -channel activity. (4–8) This then suggests that the process of Ca2+ release from the sarcoplasmic reticulum (SR) takes place at the level of the junctional foot. What is still not clear, however, is the relationship between the T-tubule and the junctional foot. The experiments described in this chapter represent an approach to the understanding of the constituents of the T-tubule and of the junction which forms the full triadic junctional structure.

Keywords

Sarcoplasmic Reticulum Ryanodine Receptor Inositol Trisphosphate Dihydropyridine Receptor Calcium Release Channel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Anthony H. Caswell
    • 1
  • Neil R. Brandt
    • 1
  • Shu-Rong Wen
    • 1
    • 2
  • Jane A. Talvenheimo
    • 1
  1. 1.Department of PharmacologyUniversity of Miami School of MedicineMiamiUSA
  2. 2.Department of PharmacologyBeijing Medical UniversityBeijingPeople’s Republic of China

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