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Biochemical Structure of the Dihydropyridine Receptor

  • Jane A. Talvenheimo
  • Shu-Rong Wen
  • Kyung Sook Kim
  • Anthony H. Caswell
Part of the Series of the Centro de Estudios Científicos de Santiago book series (SCEC)

Abstract

When radiolabeled dihydropyridine compounds first became available, they opened up a wide area of investigation for researchers interested in the biochemical structure of voltage-dependent Ca2+ -channels. Dihydropyridines (DHPs) were seen to bind to a high-affinity site closely associated with an important class of Ca2+ -channels (designated DHP-sensitive Ca2+ -channels), and provided the first tools for identifying and extracting the Ca2+ -channel protein from membranes. Investigations of the DHP recep-tor/Ca2+ -channel protein have focused on three different approaches for identifying the high-affinity DHP-binding protein: (i) radiation inactivation of the binding activity to determine the target size of the receptor, (ii) solubilization and purification of the binding activity from membranes, and (iii) photoaffinity labeling of the drug receptor in intact membranes. Nearly all of these studies have been performed using skeletal muscle transverse tubule membranes (T-tubules), a well-characterized membrane fraction(1) as a source of DHP receptors. T-tubule membranes contain the highest density of DHP binding sites (from 10 to 100 pmol of binding sites per mg membrane protein) of any tissue surveyed for DHP-binding activity. The goal of this chapter is to briefly review what is known about the protein structure of the T-tubule DHP receptor/Ca2+ -channel, to present some data on the purification and reconstitution of the DHP receptor, and to highlight some of the unanswered questions regarding the structure and possible functional role of this protein.

Keywords

Skeletal Muscle Conductance Level Planar Lipid Bilayer Rabbit Skeletal Muscle Frog Skeletal Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Jane A. Talvenheimo
    • 1
  • Shu-Rong Wen
    • 1
    • 2
  • Kyung Sook Kim
    • 1
  • Anthony H. Caswell
    • 1
  1. 1.Department of PharmacologyUniversity of Miami School of MedicineMiamiUSA
  2. 2.Department of PharmacologyBeijing Medical UniversityBeijingPeople’s Republic of China

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