Sodium Pump in T-Tubules of Frog Muscle Fibers

  • R. A. Venosa
Part of the Series of the Centro de Estudios Científicos de Santiago book series (SCEC)


It is well known that in the cytosol of most cells the high concentration of K+ ([K +] i ) and the relatively low concentration of Na+ ([Na+] i ) are kept constant in spite of their electrochemical gradients, which promotes the loss of K+ and the gain of Na+. The steadiness of [K +], and [Na+] i , is maintained by a metabolic energy-dependent active transport process first proposed by Dean(1) in skeletal muscle and known as the Na+ pump (Dean coined the name) or more properly as the Na + /K + pump.


Surface Membrane Fiber Volume Plasma Membrane Vesicle Rabbit Skeletal Muscle Frog Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Dean, R. B., 1941, Theories of electrolyte equilibrium in muscle, Cold Spr. Harb. Symp. Quant. Biol. 3: 331.Google Scholar
  2. 2.
    Clausen T., 1986, Regulation of active Na + /K+ transport in skeletal muscle, Phys. Rev. 66:542–580.Google Scholar
  3. 3.
    Skou, J. C., 1957, The influence of some cations on an adenosine triphosphatase from peripheral nerves, Biochim. Biophys. Acta 23: 394–401.PubMedCrossRefGoogle Scholar
  4. 4.
    Skou, J. C., 1960, Further investigations of Mg2 + + Na+ = activated adenosinetriphosphatase, possibly related to the active, linked transport of Na+ and K+ across the nerve membrane, Biochim. Biophys. Acta 42: 6–23.CrossRefGoogle Scholar
  5. 5.
    Horowicz, P., Taylor, J. W., and Waggoner, D. M., 1970, Fractionation of sodium efflux in frog sartorius muscle by strophanthidin and removal of external sodium, J. Gen. Physiol. 55: 401–425.PubMedCrossRefGoogle Scholar
  6. 6.
    Erlij, D., and Grinstein, S., 1976, The number of sodium ions pumping sites in skeletal muscle and its modification by insulin, J. Physiol. 259: 13–31.PubMedGoogle Scholar
  7. 7.
    Venosa, R. A., and Horowicz, P., 1981, Density and apparent location of sodium pump in frog sartorius muscle, 59: 225–232.Google Scholar
  8. 8.
    Howell, J. N., and Jenden, D. J., 1967, T-tubules of skeletal muscle: Morphological alterations which interrupt excitation-contraction coupling, Fed. Proc. 26: 553.Google Scholar
  9. 9.
    Jaimovich, E., Venosa, R. A., Shrager, P., and Horowicz, P., 1976, The density and distribution of tetrodotoxin receptors in normal and “detubulated” frog sartorius muscle, J. Gen. Physiol. 67: 399–416.PubMedCrossRefGoogle Scholar
  10. 10.
    Narahara, H. T., Vogrin, V. G., Green, J. D., Kent, R. A., and Gould, M. K., 1979, Isolation of plasma membrane vesicles, derived from transverse tubules, by selective homogenization of subcellular fractions of frog skeletal muscle in isotonic media, Biochim. Biophys. Acta 552: 247–261.PubMedCrossRefGoogle Scholar
  11. 11.
    Lau, Y. H., Caswell, A. H., Garcia, M., and Letelier, L., 1979, Ouabain binding and coupled sodium, potassium, and chloride transport in isolated transverse tubules of skeletal muscle, J. Gen. Physiol. 74: 335–349.PubMedCrossRefGoogle Scholar
  12. 12.
    Clausen, T., and Hansen, O., 1974, Ouabain binding and Na + /K+ transport in rat muscle cells and adipocytes, Biochim. Biophys. Acta 345: 387–404.CrossRefGoogle Scholar
  13. 13.
    Seiler, S., and Fleischer, S., 1982, Isolation of plasma membrane vesicles from rabbit skeletal muscle and their use in ion transport studies, J. Biol. Chem. 257:13862–13871.PubMedGoogle Scholar
  14. 14.
    Lau, Y. H., Caswell, A. H., and Brunschwig, J. P., 1977, Isolation of transverse tubules by fractionation of skeletal muscle, J. Biol. Chem. 252: 5565–6574.PubMedGoogle Scholar
  15. 15.
    Moczydlowski, E. G., and Latorre, R., 1983, Saxitoxin- and Ouabain-binding activity of isolated skeletal muscle membrane as indicator of surface origin and purity, Biochim. Biophys. Acta 732: 412–420.PubMedCrossRefGoogle Scholar
  16. 16.
    Hidalgo, C., Parra, C., Riquelme, G., and Jaimovich, E., 1986, Transverse tubules from frog skeletal muscle: Purification and properties of vesicles sealed with the inside-out orientation, Biochim. Biophys. Acta 855: 79–88.PubMedCrossRefGoogle Scholar
  17. 17.
    Jaimovich, E., Donoso, P., Liberona, J. L., and Hidalgo, C., 1986, Ion pathways in transverse tubules: Quantification of receptors in membranes isolated from frog and rabbit skeletal muscle, Biochim. Biophys. Acta 855: 89–98.PubMedCrossRefGoogle Scholar
  18. 18.
    Dulhunty, A. F., and Franzini-Armstrong, C., 1975, The relative contribution of the folds and caveolae to the surface membrane of frog skeletal muscle fibers at different sarcomere lengths, J. Physiol. 250: 513–539.PubMedGoogle Scholar
  19. 19.
    Franzini-Armstrong, C., Venosa, R. A., and Horowicz, P., 1973, Morphology and accessibility of the transverse tubular system in frog sartorius muscle after glycerol treatment, J. Membrane Biol. 14: 197–212.CrossRefGoogle Scholar
  20. 20.
    Venosa, R. A., 1978, Stimulation of the Na+ pump by hypotonic solutions in skeletal muscle, Biochim. Biophys. Acta 510: 378–383.PubMedCrossRefGoogle Scholar
  21. 21.
    Eisenberg, R. S., Howell, J. N., and Vaughan, P. C., 1971, The maintainance of the resting potentials in glycerol-treated muscle fibers, J. Physiol. 215: 95–102.PubMedGoogle Scholar
  22. 22.
    Venosa, R. A., and Horowicz, P., 1973, Effects on sodium efflux of treating frog sartorius muscle with hypertonic glycerol solutions, J. Membrane Biol. 14: 33–56.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • R. A. Venosa
    • 1
  1. 1.Cátedra de Fisiología y Biofísica, Facultad de Ciencias MédicasUniversidad Nacional de La PlataLa PlataArgentina

Personalised recommendations