Dermatologic Antioxidant Therapy may be Warranted to Prevent Ultraviolet Induced Skin Damage
Experimental and clinical evidence is increasing that free radical processes are implicated in various pathological conditions in skin, such as ultraviolet and ionizing irradiation damage, thermal trauma, phototoxicity and photoallergy, drug toxicity, skin aging, skin autoimmune disease and tumor promotion. Direct evidence for ultraviolet induced free radical formation in skin has been obtained by low temperature electron paramagnetic resonance spectroscopy (Norrins 1962, Pathak 1968). It was suggested that there is no direct evidence relating formation of reactive oxygen species with acute or chronic ultraviolet effects (Epstein 1977). However, a considerable body of circumstantial evidence has been ammassed that strongly inferes that reactive oxygen species or reactions initiated by them are responsible for at least some of the deleterious effects of ultraviolet upon skin (Black 1987). Exposure of human and animal skin to UVB (280–320 nm) and UVA (320–400 nm) irradiation results in a significant elevation of lipid peroxidation products. Lipid peroxides are toxic in mammalian skin; epidermal damage due to epicutaneous application of lipid peroxides could be mediated by propagation of lipid peroxidation in situ. Injection of an aqueous extract of ultraviolet irradiated linoleic acid into skin induces necrosis of the epidermis and dermis followed by inflammatory response and disruption of collagen and elastic fibres (Waravdekar 1965).
KeywordsElectron Spin Resonance Free Radical Scavenge Activity Hairless Mouse Sunburn Cell Skin Excisates
Unable to display preview. Download preview PDF.
- Del Rio LA, Ortega MG, Lopez AL, Gorge XL. A more sensitive modification of the catalase assay with the clark oxygen electrode. Methods Enzymol. 105, 409–415, 1977.Google Scholar
- Epstein JH. The pathological effects of light on the skin. In:“Free radicals in biology” , Pryor WA. ed, Academic Press, New York, pp 219– 249, 1977.Google Scholar
- Fuchs J, Huflejt ME, Rothfuss LM, Wilson DS, Carcamo G, Packer L. Impairment of enzymic and non-enzymic antioxidants in skin by UVB irradiation. To be published, 1989a.Google Scholar
- Fuchs J, Mehlhorn RJ, Packer L. Free radical reduction mechanisms in mouse epidermis and skin homogenates. To be published, 1989b.Google Scholar
- Msika P, Cesarini JP, Poelman MC. Antioxidants and aggressions from ultraviolet radiation in man. Society for Free Radical Research Winter Meeting on“ Free Radicals in Medicine: Current status of antioxidant therapy”, Paris, December 8–9, 1988.Google Scholar
- Norrins AL. Free radical formation in the skin following exposure to ultraviolet light. J Invest Dermatol 39, 445–448, 1962.Google Scholar
- Waravdekar VS, Saslaw LD, Jones WA, Kuhns JG. Skin changes induced by UV-irradiated linoleic acid extract. Arch Pathol 80, 91–95,1965.Google Scholar