Reactivity of Active Centre Analogues of Cu2Zn2Superoxide Dismutase
Active centre analogues of Cu2Zn2superoxide dismutase were devised and successfully employed. Emphasis was placed on the flexible nature of the Superoxide mimicking compounds. Di-Schiff-bases proved most appropri ate to fulfil these requirements. Both structural and functional aspects of the copper binding centre of the intact enzyme were met by these complexes. Nanomolar concentrations of copper coordinated in these complexes were sufficient to inhibit the K3CrO8 induced chemiluminescence identical to the reaction of Cu2Zn2superoxide dismutase.
KeywordsSuperoxide Dismutase Hyaluronic Acid Superoxide Dismutase Activity Copper Chelate Nitro Tetrazolium
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