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Iron Redistribution and Lipid Peroxidation in the Cold Ischaemic Kidney

  • Jon D. Gower
  • Guy Healing
  • Barry J. Fuller
  • Colin J. Green
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 264)

Abstract

Oxygen-derived free radicals may play an important role in the damage which occurs to organs subjected to extended periods of cold storage followed by reperfusion with oxygenated blood upon transplantation into the recipient1. One damaging radical-mediated process is the peroxidation of membrane-bound polyunsaturated fatty acids and we have previously demonstrated significant elevations in lipid peroxidation markers in kidneys subjected to cold storage and autotransplantation2. Iron is required for the initiation of lipid peroxidation3 which may be the consequence of highly reactive OH. radical formation from O 2 .- and H2O2 via the Haber-Weiss reaction or direct attack on polyunsaturated fatty acids by iron complexes with oxygen4. In addition, iron also catalyses the decomposition of lipid hydroperoxides (LOOH) to alkoxy (LO.) and peroxy (LOO.) radicals which stimulate the chain reaction of lipid peroxidation4. In order to minimize the likelihood of these damaging reactions, iron is transported and stored in specific proteins. However, a small pool of iron exists in the cell as low molecular weight chelates5 which are able to exert catalytic activity.

Keywords

Cold Storage Chelatable Iron Cold Ischaemia Rabbit Kidney Total Iron Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B.J. Fuller, J.D. Gower, and C.J. Green, Free radical damage and organ preservation: fact or fiction?, Cryobiology 25:377 (1988).PubMedCrossRefGoogle Scholar
  2. 2.
    C.J. Green, G. Healing, J. Lunec, B.J. Fuller, and S. Simpkin, Evidence of free radical-induced damage in rabbit kidneys after simple hypothermic preservation and auto transplantation, Transplantation 41:161 (1986).,PubMedCrossRefGoogle Scholar
  3. 3.
    E.D. Wills, Mechanisms of lipid peroxide formation in animal tissues, Biochem. J. 99:667 (1966).PubMedGoogle Scholar
  4. 4.
    J.M.C. Gutteridge, B. Halliwell, and D.A. Rowley, Catalytic iron complexes in biological material: A potential for oxygen radical damage, Life Chem. Rep. 1, Suppl 2:15 (1981).Google Scholar
  5. 5.
    D.L. Bakkeren, C.M.H. de Jeu-Jaspars, C. van der Heul, and H.G. van Eijk, Analysis of iron-binding components in the low molecular wei¿£it fraction of rat reticulocyte cytosol, Int. J. Biochem. 17:925 (1985).PubMedCrossRefGoogle Scholar
  6. 6.
    G. Healing, J.D. Gower, B.J. Fuller, and C.J0 Green, Release of chelatable iron and free radical damage to rabbit kidneys subjected to ischaemia and reperfusion, Med. Sci. Res. 17:67 (1989).Google Scholar
  7. 7.
    J. Gower, G. Healing, and C. Green, Measurement by hplc of desferrioxamine-available iron in rabbit kidneys to assess the effect of ischaemia on the distribution of iron within the total pool, Free Rad. Res. Comms. 5:291 (1989).CrossRefGoogle Scholar
  8. 8.
    O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall, Protein measurement with the folin phenol reagent, J. Biol. Chem. 192:265 (1951).Google Scholar
  9. 9.
    B.J. Fuller, J. Lunec, G. Healing, S. Simpkin, and C.J. Green, Reduction of susceptibility to lipid peroxidation by desferrioxamine in rabbit kidneys subjected to 24-hr cold ischaemia and reperfusion, Transplantation 43:604 (1987).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Jon D. Gower
    • 1
  • Guy Healing
    • 1
  • Barry J. Fuller
    • 2
  • Colin J. Green
    • 1
  1. 1.Section of Surgical ResearchClinical Research CentreHarrow, MiddlesexUK
  2. 2.Academic Department of SurgeryRoyal Free Hospital School of MedicineHampstead, LondonUK

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