Inhibition of Bleomycin-Induced Toxic Effects by Antioxidants in Human Malignant Melanoma Cells

  • H. Kappus
  • Ch. Reinhold
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 264)


Bleomycin is an antibiotic drug used in tumor chemotherapy. It forms a complex with iron ions which can be reduced chemically or enzymatically. The bleoraycin-Fe(II)-complex binds and activates oxygen and degrades cellular DNA. This activity of bleomycin is presumably responsible for the cytotoxic effects observed during therapy (for reviews see Burger et al., 1981; Hecht, 1986). Because reactive oxygen species are involved, we wondered whether antioxidants could inhibit bleomycin-induced cytotoxicity. For example, the seleno-organic compound Ebselen (2-pheny1–1,3-benzisoselenazo1–3(2H)one) has besides its glutathione peroxidase-like activities also antioxidant properties (Müller et al., 1984). It inhibits cell toxicity induced by adriamycin (Doroshow, 1986) and diquat (Cotgreave et al., 1987), both redox cycling compounds which can induce formation of reactive oxygen species. The other antioxidants examined were pyrogallol and propyl gallate which have also been shown to trap reactive oxygen species (Weinke et al., 1987).


Human Malignant Melanoma Cell Redox Cycling Propyl Gallate Significant Toxic Effect Strand Scission 
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  1. Burger, R.M., Peisach, J., and Horwitz, S.B., 1981, Activated bleomycin. A transient complex of drug, iron, and oxygen that degrades DNA, J. Biol. Chem. 256:11636Google Scholar
  2. Cotgreave, I.A., Sandy, M.S., Berggren, M., Moldeus, P.W., and Smith, M.T., 1987, N-Acetýlcysteine and glutathione-dependent protective effect of PZ5l (ebselen) against diquat-induced cytotoxicity in isolated hepatocytes, Biochem. Pharmacol. 36:2899PubMedCrossRefGoogle Scholar
  3. Doroshow, J.H., 1986, Role of hydrogen peroxide and hydroxyl radical formation in the killing of Ehrlich tumor cells by anticancer guinones, Proc. Nat. Acad. Sci. USA 83:4514PubMedCrossRefGoogle Scholar
  4. Hecht, S.M., 1986, DNA strand scission by activated bleomycin group antibiotics, Fed. Proc. 45:2784PubMedGoogle Scholar
  5. Kappus, H., Mahmutoglu, I., Kostrucha, J., and Scheulen, M.E., 1987, Liver nuclear NADPH-cytochrome P-450 reductase may be involved in redox cycling of bleomycin-Fe(III), oxy radical formation and DNA damage, Free Rad. Res. Comms. 2:271Google Scholar
  6. Kilkuskie, R.E., MacDonald, T.L., and Hecht, S.M., 1984, Bleomycin may be activated for DNA cleavage by NADPH-cytochrome P-450 reductase, Biochemistry 23:6165PubMedCrossRefGoogle Scholar
  7. Mahmutoglu, I., and Kappus, H., 1985, Oxy radical formation during redox cycling of the bleomycin-iron (III) complex by NADPH-cytochrome P-450 reductase, Biochem. Pharmacol. 34:3091PubMedCrossRefGoogle Scholar
  8. Mahmutoglu, I., and Kappus, H., 1987, Redox cycling of bleomycin-Fe(III) by an NADH-dependent enzyme and DNA damage in isolated rat liver nuclei, Biochem. Pharmacol. 36:3677PubMedCrossRefGoogle Scholar
  9. Mahmutoglu, I., and Kappus, H., 1988, Redox cycling of bleomycin-Fe(III) and DNA degradation by isolated NADH-cytochrome b5 reductase -Involvement of cytochrome b5. Mol. Pharmacol. 34:578PubMedGoogle Scholar
  10. Müller, A., Cadenas, E., Graf, P., and Sies, H., 1984, A novel biologically active seleno-organic compound I. Glutathione peroxidase-like activity in vitro and antioxidant capacity of PZ5l (Ebselen). Biochem. Pharmacol. 33:3235Google Scholar
  11. Scheulen, M.E., Kappus, H., Thyssen, D., and Schmidt, C.G., 1981, Redox cycling of Fe(III)-bleomycin by NADPH cytochrome P-450 reductase, Biochem. Pharmacol. 30:3385PubMedCrossRefGoogle Scholar
  12. Wendel, A., Otter., R., and Tiegs, G., 1986, Inhibition by Ebselen of microsomal NADPH-cytochrome P-450 reduc tase in vitro but not in vivo, Biochem. Pharmacol. 35:2995PubMedCrossRefGoogle Scholar
  13. Weinke, S., Kahl, R., and Kappus, H., 1987, Effect of four synthetic antioxidants on the formation of ethylene from methional in rat liver microsomes, Toxicol. Letters 35:247CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • H. Kappus
    • 1
  • Ch. Reinhold
    • 1
  1. 1.Department of Dermatology, Rudolf-Virchow-ClinicFree University of BerlinBerlin 65Germany

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