Selenium and Glutathione Peroxidase Activity in Diploid Human Fibroblasts: Their Effects Against H2O2 or UVB Induced Toxicity

  • M. J. Richard
  • P. Frappat
  • J. Arnaud
  • A. Favier
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 264)


One of the proposed mechanisms of aging is generation of free radicals that have been shown to react with lipids, proteins, and DNA1.2. Trace elements by the fact of metalloenzymes (Zinc Copper Superoxide Dismutase (ZnCuSOD), Man ganese Superoxide Dismutase (MnSOD), Selenium Glutathione Peroxidase (SeGPX)) prevent such potentially toxic reactions.


Sodium Selenite Glutathione Peroxidase Activity Diploid Human Fibroblast Zinc Copper Superoxide Butyl Hydroperoxide 
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  1. 1.
    S.K. Jain, Evidence for membrane lipid peroxidation during the in vivo aging of human erythroeytes, Biochim.bipphys. 6c.ta..-937 : 205–210 ( 1988 ) .CrossRefGoogle Scholar
  2. 2.
    T.F. Slater, Free radical mechanisms in tissue injury. Bipchem. J., 222:1–15 (1984).Google Scholar
  3. 3.
    K. Takahashi, P.E. Newburger, and H.J. Cohen, Glutathione peroxidase protein: Absence in selenium deficiency states and correlation with enzymatic activity. J. Clin. Invest. 77:1402–1404 (1986)PubMedCrossRefGoogle Scholar
  4. 4.
    G.F. Combs and S.B. Combs, in : “The role of Selenium in nutrition,” Combs & Combs, Ed., Academic Press Inc. London (1986) pp 205–264.Google Scholar
  5. 5.
    W.A.Gunzler, H.Kremers, and L. Flohe. An improved coupled test procedure for glutathione peroxidase in blood. Z. Klin. Chem. Klin. Biochem. 12:444– 448 (1974).PubMedGoogle Scholar
  6. 6.
    S.B. Dousset, M. Trouilh, and M.J. Foglietti, Plasma malonaldehyde levels during myocardial infarction. Clin. Chim. Acta. 129:319–322(1983).PubMedCrossRefGoogle Scholar
  7. 7.
    Ch. Shopsis, G.J. Mackay, Semi automated assay for cell culture. Anal. Bipchem. 140:104–107 (1984).CrossRefGoogle Scholar
  8. 8.
    Ao Pung , liei Zhao, and Yu Shu-yu, In vitro differential effects of sodium selenite on the growth of human hepatoma cells and human embryonic liver cells. Bio. Trace Elem. Res. 14:1–17 (1987).CrossRefGoogle Scholar
  9. 9.
    J.F. Mead, Free radical mechanisms of lipid damage and conseguences for cellular membranes. in: “Free Radicals in Biology,” W.A. Pryor, ed., Academic Press.N.Y. (1976) 1: pp 51–66.Google Scholar
  10. 10.
    D. Herman, Free radicals in aging, Mol. Cell. Bipchem. 84:155–161 (1988).CrossRefGoogle Scholar
  11. 11.
    J. Chaudière, D. Gerard, M. Clement, J.M. Bourre. Induction of Selenium glutathione peroxidase by stimulation of metabolic hydrogen peroxide production in vivo. Bipchem. Bioenergetics 18:247–256 (1987)CrossRefGoogle Scholar
  12. 12.
    E. Hoffman, A.C. Mello Filho, and R. Meneghmi, Correlation between cytotoxic effect of hydrogen peroxide and the yield of DNA strand breaks in cells of different species, Biochim. bipphys. Acta. 781:234–238 (1984).Google Scholar
  13. 13.
    E.B. Thorling, K. Overvad, and P. Bjerring, Oral selenium inhibits skin reactions to UV light in hairless mice. Acta. Path. Micrpbiol. Immunol. Scand. Sect. A., 91:81–83 (1983).Google Scholar
  14. 14.
    D. Medina, H.W. Lane, and C.M. Tracey, Selenium and mouse mammary tumorigenesis : an investigation of possible mechanisms. Cancer Res. suppl.43: 2460–2464 (1983).Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • M. J. Richard
    • 1
  • P. Frappat
    • 1
  • J. Arnaud
    • 1
  • A. Favier
    • 1
  1. 1.Laboratoire de Biochimie CC.H.R.U.G.Grenoble CédexFrance

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