Extracellular-Superoxide Dismutase, Distribution in the Body and Therapeutic Applications

  • Stefan L. Marklund
  • Kurt Karlsson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 264)


Extracellular-superoxide dismutase (EC, EC-SOD) is a secretory, tetrameric, Cu and Zn-containing glycoprotein with a subunit molecular weight of about 30 kDa.1,2 EC-SOD is the major SOD isoenzyme in extracellular fluids.3,4 Although it is the least predominant SOD isoenzyme in the tissues, 90 to 99 % of the EC-SOD in the bogy of mammals is located to the extravascular space of tissues.5,6


Heparan Sulfate Subunit Molecular Weight Creatine Kinase Release Potassium Superoxide Oxygen Radical Research 
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  1. 1.
    S.L. Marklund, Human copper-containing Superoxide dismuta-se of high molecular weight, Proc. Natl. Acad. Sci. USA. 79:7634–7638 (1982).PubMedCrossRefGoogle Scholar
  2. 2.
    L. Tibell, K. Hjalmarsson, T. Edlund, G. Skogman, Å. Engström and S.L. Marklund, Expression of human extracellular-superoxide dismutase in Chinese hamster ovary cells and characterization of the product, Proc. Natl. Acad. Sci. USA. 84:6634–6638 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    S.L. Marklund, E. Holme and L. Hellner, Superoxide dismutase in extracellular fluids, Clin. Chim. Acta, 12 6: 41–51 (1982).CrossRefGoogle Scholar
  4. 4.
    S.L. Marklund, A. Bjelle and L.-G. Elmqvist, Superoxide dismutase isoenzymes of the synovial fluid in rheumatoid arthritis and in reactive arthritides, Ann. Rheum. Dis. 45:847–851 (1986).PubMedCrossRefGoogle Scholar
  5. 5.
    S.L. Marklund, E×tracellular-superoxide dismutase in human tissues and human cell lines, J. Clin. Invest. 74: 1398–1403 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    S.L. Marklund, Extracellular-superoxide dismutase and other Superoxide dismutase isoenzymes in tissues from nine mammalian species, Biochem. J. 222:649–655 (1984).PubMedGoogle Scholar
  7. 7.
    K. Karlsson and S.L. Marklund, Heparin-induced release of extracellular-superoxide dismutase to human blood plasma, Biochem. J. 242:55–59 (1987).PubMedGoogle Scholar
  8. 8.
    K. Karlsson and S.L. Marklund, Extracellular-superoxide dismutase in the vascular system of mammals. Biochem. J. 255:223–228 (1988).PubMedGoogle Scholar
  9. 9.
    S.L. Marklund, EC-SOD, molecular composition and distribution in the body, in: Oxidative damage and related enzymes, G. Rotilio and J.V. Bannister, eds. Life Chemistry Reports Supplement Series, Harwood Academic Publishers, pp. 411–416 (1984).Google Scholar
  10. 10.
    K. Hjalmarsson, S.L. Marklund, Å. Engström and T. Edlund, Isolation and sequence of complementary DNA encoding human extracellular-superoxide dismutase, Proc. Natl. Acad. Sci. USA. 84:6340–6344 (1987).PubMedCrossRefGoogle Scholar
  11. 11.
    K. Karlsson and S.L. Marklund, Plasma clearance of human extracellular-superoxide dismutase C in rabbits, J. Clin. Invest. 82:762–766 (1988).PubMedCrossRefGoogle Scholar
  12. 12.
    K. Karlsson and S.L. Marklund, Heparin-, dextran sulfate-and protamine-induced release of extracellular-superoxide dismutase to plasma in pigs, Biochim. Biophys. Acta. 967:110–114 (1988).PubMedCrossRefGoogle Scholar
  13. 13.
    K. Karlsson and S.L. Marklund, Binding of human extracellular-superoxide dismutase C to cultured cell lines and to blood cells, Lab. Invest, 6:659–666 (1989).Google Scholar
  14. 14.
    S.L. Marklund, Direct assay with potassium Superoxide, in: Handbook of Methods for Oxygen Radical Research, R. Greenwald, ed. CRC press, pp. 249–255 (1985).Google Scholar
  15. 15.
    J.M. McCord and Fridovich, I, J. Biol. Chem. 244:6049– 6055 (1969).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Stefan L. Marklund
    • 1
  • Kurt Karlsson
    • 1
  1. 1.Department of Clinical ChemistryUmeå University HospitalUmeåSweden

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