Expression of Herpes Simplex Virus 1 Glycoprotein B in Human Cells and Protection of Mice against Lethal Herpes Simplex Virus 1 Infection
Expression of cloned viral and cellular genes in eukaryotic cells is relevant to production of vaccines (Melnick, 1986; Moss and Flexner, 1987) and gene therapy (Anderson, 1984). To this purpose numerous eukaryotic vectors have been developed. Since the level of expression of exogenous genes is directly related to the degree of gene amplification (Rigby, 1983) episomal eukaryotic vectors have been constructed where a viral origin of replication directs vector amplification in suitable cells (DiMaio, 1987; Milanesi et al., 1984). However, only two eukaryotic vectors (Milanesi et al., 1984; Sugden et al., 1985) are specific for human cells and they allow expression of genes endowed with their own regulatory sequences. We have therefore devised a viral vector (pBK-1) that replicates and persists episomally in human cells; The structure and general characteristics of this vector have been described in detail (Milanesi et al., 1984). It contains DNA sequences from the plasmid pML, a deletion derivative of pBR322, as well as the origin of replication and the early region of BK virus (BKV), a human papovavirus that efficently replicates in human cells.
KeywordsHerpes Simplex Virus Type North Atlantic Treaty Organization Vector Copy Number Herpes Simplex Virus Glycoprotein Eukaryotic Vector
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