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Different Expression of HLA Class I Antigens in Liver of Children with Chronic Hepatitis B, Evaluated by Immunohistochemical Method

  • R. Calzia
  • M.G. Marazzi
  • A. Campelli
  • R. Piscopo
  • M. Dorati
  • F. Puppo
  • L. Borgiani
  • M. Canepa
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 257)

Summary

Studies on the quantitative expression of the Major Histocompatibility Complex (MCH) in hepatocytes chronically infected by Hepatitis B Virus (HBV) report that an increased expression of these antigens could be related to a good immunological response.

In the present work we analyze the expression of the MCH antigens in cryostatic sections of liver biopsies taken from subjects (1 9 children) with various forms o f HBsAg positive chronic hepatitis.

A high expression o f HLA class I antigens and a high degree of hepatocyte necrosis was evident in Chronic Active Hepatitis (CAH) and Chronic Lobular Hepatitis (CLH). On the contrary, subjects with histological diagnosis of Chronic Persistent Hepatitis (CPH) showed a low expression of such antigens. There was however, the difference that in subjects with high hepatic cytolysis and high expression o f HLA class I antigens , serum HBV-DNA was clearly present in almost all the cases with CAH, but not detectable in all cases with CLH.

The expression of HLA class II antigens and of Beta2 microglobulin was the same in all 19 cases. All cases with HBV-DNA positivity with high class I antigen expression had active hepatitis which seems to suggest that all attemps at viral clearance on the part of the immune system have been in vain. We hope our paper will be an additional parameter for evaluating the course of hepatitis during Interferon treatment.

Keywords

Major Histocompatibility Complex Chronic Hepatitis Chronic Active Hepatitis Hepatitis Delta Virus Chronic Persistent Hepatitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    G. Ballardini, F.B. Bianchi, R. Mirakian, M. Fallani, E. Pisi, G.F. Bottazzo, 1987, HLA-A,B,C, HLA-D/DR and HLA-D/DQ expression on unfixed liver biopsy sections from patients with chronic liver disease. Clin. Exp. Immunol. 70, 35–46.PubMedGoogle Scholar
  2. 2.
    C. Chia-Ming, S. Wei-Chue, K. Ruey-Wen, L. Yun-Fan, 1987, HLA class I antigen display on hepatocyte membrane in chronic hepatitis B virus infection: its role in the pathogenesis of chronic type B hepatitis. Hepatology 7, 6: 1311–1316.CrossRefGoogle Scholar
  3. 3.
    R. De Vos, C. De Wolf-Peters, J. Van den Oord and V. Desmet, 1985, Ultrastructural immunocytochemical demonstration of HLA class I antigens in human pathological liver tissue. Hepatology 5, 6: 1071–1075.PubMedCrossRefGoogle Scholar
  4. 4.
    M. Igarashi, L. Imberti, M. Maio, M. Tsujisaki, F. Perosa, K. Sakaguchi, P.G. Natali, S. Ferrone, 1986, Preparation characterization, and utilization of monoclonal antibodies to the gene products of the HLA-D region with special enphasis on those to polymorphic determinants. In “HLA class II antigens” pagg. 224–248 B.G. Solheim, E. Moller, S. Ferrone eds. Springer-Verlag — Berlin.CrossRefGoogle Scholar
  5. 5.
    L. Montano, G.C. Miescher, A.H. Goodali, K.H. Wiedmann, G. Janossi, H.C. Thomas 1982, Hepatitis B virus and HLA antigen display in the liver during chronic hepatitis B virus infection. Hepatology 2, 5: 557–561.PubMedCrossRefGoogle Scholar
  6. 6.
    Y. Nagafuchi, P. Scheuer, 1986, Expression of Beta 2-microglobulin on hepatocytes in acute and chronic type B hepatitis. Hepatology 6, 1: 20–23.PubMedCrossRefGoogle Scholar
  7. 7.
    R.G. Paul, S.T. Roodman, D.A. Paul, R.P. Perrillo, 1987, Elevated HLA class I antigen expression on peripheral blood mononuclear cells of HbsAg carriers with coexistent human immunodeficiency virus infection. Hepatology 7, 6: 1326–1328.PubMedCrossRefGoogle Scholar
  8. 8.
    M. Pignatelli, J. Waters, D. Brown, A. Lever, S. Iwarson, Z. Shaff, R. Gerety, H.C. Thomas, 1986, HLA class I antigens on the hepatocyte membrane during recovery from acute hepatitis B virus infection and during Interferon therapy in chronic hepatitis B virus infection. Hepatology 6, 3: 349–353.PubMedCrossRefGoogle Scholar
  9. 9.
    H.C. Thorns, M. Pignatelli, 1985, Is modulation of HLA display by Interferon important in preventing the development of the chronic carrier state of hepatitis B virus in adults? Gastroenterol. Clin. Biol. 9: 287–289.Google Scholar
  10. 10.
    J .J. Van den Oord, V.J. Desmet, 1984, Distribution patterns of major histocompatibility antigens in normal and diseased liver tissue. Leber, Magen, Darm 14: 244–254.PubMedGoogle Scholar
  11. 11.
    J.J. Van den Oord, R. De Vos, V.J. Desmet, 1986, In situ distribution of major histocompatibility complex products and viral antigens in chronic hepatitis B virus infection: evidence that HBc-containing hepatocytes may express HLA-DR antigens. Hepatology 6, 5: 981–986.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • R. Calzia
  • M.G. Marazzi
    • 1
  • A. Campelli
    • 1
  • R. Piscopo
    • 1
  • M. Dorati
  • F. Puppo
  • L. Borgiani
    • 2
  • M. Canepa
    • 2
  1. 1.Dept. of Internal Medicine and 2nd Clinic Infectious DiseaseUniversity of GenoaGenovaItaly
  2. 2.Dept. of PathologyGalliera Hospital GenovaGenovaItaly

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