The Analysis of Immunohistochemical Data
Neuroscientists have been interested for many years in the anatomic distribution and concentration of neurochemicals in the brain. Particular attention has focused on the localization of neurotransmitters. Biochemical techniques are now used routinely to assay for the concentration, uptake, or evoked release of neurotransmitters in brain. Microdissection techniques allow one to make these measurements in relatively small brain regions. However, the spatial resolution of biochemical assays is extremely coarse relative to the anatomic complexity of the brain, and it has rarely been possible to measure concentrations at the level of single cells and fibers.
KeywordsGray Level Lateral Geniculate Nucleus Antibody Label Integrate Optical Density Average Optical Density
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For Further Reading
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