The Analysis of Immunohistochemical Data

  • R. Ranney Mize


Neuroscientists have been interested for many years in the anatomic distribution and concentration of neurochemicals in the brain. Particular attention has focused on the localization of neurotransmitters. Biochemical techniques are now used routinely to assay for the concentration, uptake, or evoked release of neurotransmitters in brain. Microdissection techniques allow one to make these measurements in relatively small brain regions. However, the spatial resolution of biochemical assays is extremely coarse relative to the anatomic complexity of the brain, and it has rarely been possible to measure concentrations at the level of single cells and fibers.


Gray Level Lateral Geniculate Nucleus Antibody Label Integrate Optical Density Average Optical Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

For Further Reading

  1. Brown, C. (1977). Neuron orientations: A computer application. In: Computer Analysis of Neuronal Structures ( R. D. Lindsay, ed.). New York: Plenum Press, pp. 177–188.CrossRefGoogle Scholar
  2. McEachron, D. (1986). Function Mapping in Biology and Medicine: Computer Assisted Autoradiography. Basel: Karger.Google Scholar
  3. Mize, R. R., ed. (1985). The Microcomputer in Cell and Neurobiology Research. New York: Elsevier.Google Scholar
  4. Gonzales, R. C., and P. W. Wintz (1977). Digital Image Processing. Reading, MA: Addison-Wesley.Google Scholar
  5. Pratt, W. K. (1978). Digital Image Processing. New York: John Wiley & Sons.Google Scholar
  6. Castleman, K. R. (1979). Digital Image Processing. Englewood Cliffs, NJ: Prentice - Hall.Google Scholar
  7. Rosenfeld, A., and A. C. Kak (1982). Digital Imaging Processing, 2nd ed. New York: Academic Press.Google Scholar
  8. Agnati, L. F., K. Fuxe, F. Benfenati, I. Zini, M. Zoli, L. Fabbri, and A. Harfstrand (1984a). Computer-assisted morphometry and microdensitometry of transmitter-identified neurons with special reference to the mesostriatal dopamine pathway. I. Methodological aspects. Acta Physiol. Scand. [Suppl.] 532: 5–36.Google Scholar
  9. Ramm, P., and J. H. Kulick (1985). Principles of computer-assisted imaging in autoradiographic densitometry. In: The Microcomputer in Cell and Neurobiology Research ( R. R. Mize, ed.). New York: Elsevier, pp. 311–334.Google Scholar
  10. Reis, D. J., R. H. Benno, L. W. Tucker, and T. H. Joh (1982). Quantitative immunocytochemistry of tyrosine hydroxylase in brain. In: Cytochemical Methods in Neuroanatomy ( V. Chan-Palay and S. L. Palay, eds.). New York: Alan R. Liss, pp. 205–228.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • R. Ranney Mize
    • 1
  1. 1.Department of Anatomy and NeurobiologyUniversity of Tennessee Health Science CenterMemphisUSA

Personalised recommendations