Regulatory Actions of the Insulin-Like Growth Factor, IGF-I (Somatomedin-C), on Sterol Metabolism by Ovarian Cells

  • Johannes D. Veldhuis


Steroidogenic cells require large quantities of sterol substrate for utilization in the biosynthesis and secretion of relevant steroid hormones (1,2). Although the regulation of cholesterol’s economy in ovarian (granulosa and luteal) cells has been characterized extensively for gonadotropic hormones (particularly LH and HCG), fewer studies are available that delineate the nature of trophic growth factor control of sterol delivery to, and utilization by, the steroidogenic apparatus. To examine the mechanisms subserving the actions of differentiating growth factors, we have employed an in vitro serum-free culture system of swine granulosa cells that is highly responsive to the insulin-like growth factor, IGF-I (Somatomedin-C) (3,4).


Granulosa Cell Follicular Fluid Trophic Action Porcine Granulosa Cell Differentiate Growth Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Strauss JF III, Schuler LA, Rosenblum MF, Tanaka T. Cholesterol metabolism by ovarian tissue. Adv Lipid Res 1981; 18:99–127.PubMedGoogle Scholar
  2. 2.
    Gwynne JT, Strauss JF III. The role of lipoproteins in steroidogenesis and cholesterol metabolism in steroidogenic cells. Endocr Rev 1982; 3:299–321.PubMedCrossRefGoogle Scholar
  3. 3.
    Veldhuis JD, Furlanetto RW. Trophic actions of human somatomedin C/insulin-like growth factor I (IGF-I) on ovarian cells: in vitro studies using swine granulosa cells. Endocrinology 1985; 116:1235–42.PubMedCrossRefGoogle Scholar
  4. 4.
    Veldhuis JD, Demers LM. A role for somatomedin C as a differentiating hormone and amplifier of hormone action on ovarian cells: studies with synthetically pure somatomedin C and swine granulosa cells. Biochem Biophys Res Commun 1985; 130:234–40.PubMedCrossRefGoogle Scholar
  5. 5.
    Goldstein JL, Brown MS. The low-density lipoprotein pathway and its relation to atherosclerosis. Annu Rev Biochem 1977; 46:897–930.PubMedCrossRefGoogle Scholar
  6. 6.
    Veldhuis JD, Rodgers RJ, Furlanetto RW. Synergistic actions of estradiol and the insulin-like growth factor, somatomedin C, on swine ovarian (granulosa) cells. Endocrinology 1986; 119:530–8.PubMedCrossRefGoogle Scholar
  7. 7.
    Barano JLS, Hammond JM. Comparative effects of insulin and insulin-like growth factors on DNA synthesis and differentiation of porcine granulosa cells. Biochem Biophys Res Commun 1985; 124:484–91.CrossRefGoogle Scholar
  8. 8.
    Hammond JM, Baranao LS, Skleris D, Knight AB, Romanus JA, Rechler MM. Production of insulin-like growth factors by ovarian granulosa cells. Endocrinology 1985; 117:2553–7.PubMedCrossRefGoogle Scholar
  9. 9.
    Hsueh AJW, Adashi EY, Jones PBC, Welsh TH Jr. Hormonal regulation of the differentiation of cultured ovarian granulosa cells. Endocr Rev 1984; 5:76.PubMedCrossRefGoogle Scholar
  10. 10.
    Veldhuis JD, Nestler JE, Strauss JF III. The insulin-like growth factor, IGF-I (somatomedin c), modulates low-density lipoprotein metabolism by swine granulosa cells. Endocrinology 1987; 121:340–6.PubMedCrossRefGoogle Scholar
  11. 11.
    Veldhuis JD, Gwynne JT. Properties of low-density lipoprotein binding by cultured swine granulosa cells. Endocrinology 1985; 117:1067–74.PubMedCrossRefGoogle Scholar
  12. 12.
    Veldhuis JD, Gwynne JT. Estrogen regulates low-density lipoprotein metabolism by cultured swine granulosa cells. Endocrinology 1985; 117:1321–7.PubMedCrossRefGoogle Scholar
  13. 13.
    Veldhuis JD. Follicle-stimulating hormone replaces low-density lipoprotein metabolism by swine granulosa cells. Endocrinology 1988 (in press).Google Scholar
  14. 14.
    Veldhuis JD, Strauss JF III, Silavin SL, Kolp LA. The role of cholesterol esterification in ovarian steroidogenesis: studies in cultured swine granulosa cells using a novel inhibitor of acyl coenzyme A: cholesterol acyltransferase. Endocrinology 1985; 116:25–30.PubMedCrossRefGoogle Scholar
  15. 15.
    Veldhuis JD, Rodgers RJ. Mechanisms subserving the steroidogenic synergism between FSH and the insulin-like growth factor, IGF-I (somatomedin C): alterations in cellular sterol metabolism in swine granulosa cells. J Biol Chem 1987; 262:7658–64.PubMedGoogle Scholar
  16. 16.
    Veldhuis JD, Rodgers RJ, Dee A, Simpson ER. The insulin-like growth factor, somatomedin C, induces the synthesis of cholesterol side-chain cleavage cytochrome P-450 and adrenodoxin in ovarian cells. J Biol Chem 1986; 261:2499–502.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Johannes D. Veldhuis
    • 1
  1. 1.Department of Internal Medicine, School of MedicineUniversity of VirginiaCharlottesvilleUSA

Personalised recommendations