Comparison of Gonadotropin- and Catecholamine-Induced Alterations of the Adenylyl Cyclase G-Proteins in Rabbit Corpora Lutea

  • Bhanu P. Jena
  • Joel Abramowitz


It is well established that the corpus luteum in rabbits contains an adenylyl cyclase that is responsive to both LH/hCG and catecholamines (1–4), and that both these hormones activate the same adenylyl cyclase (3,4). Earlier studies have shown that exposure of corpora lutea or ovarian tissues to either LH/hCG or catecholamines leads to refractoriness of the adenylyl cyclase in these tissues (5,6), and that this refractoriness is in part reflective of changes and/or loss in receptor function (7,8). Guanine nucleotide-binding regulatory proteins (G-proteins) transduce hormone-receptor occupancy to the catalytic subunit of adenylyl cyclase, hence playing a crucial role in signal transduction (9). Therefore, the present study was undertaken to assess changes in luteal adenylyl cyclase activity and G-protein function, after injection of either hCG or epinephrine (EPI) to pseudopregnant female rabbits. In this study, we present data that show alterations in G-proteins of the rabbit luteal adenylyl cyclase system after hormone treatment.


Adenylyl Cyclase Adenylyl Cyclase Activity Heterologous Desensitization Adenylyl Cyclase System Basal Adenylyl Cyclase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Birnbaumer L, Yang PC, Hunzicker-Dunn M, Bockaert J, Duran JM. Adenylyl cyclase activities in ovarian tissues. I. Homogenization and conditions of assay in Graafian follicles and corpora lutea of rabbits, rats, and pigs: regulation by ATP, and some comparative properties. Endocrinology 1976; 99:163–84.PubMedCrossRefGoogle Scholar
  2. 2.
    Coleman AJ, Paterson DS, Somerville AR. The beta-adrenergic receptor of rat corpus luteum membranes. Biochem Pharmacol 1979; 28:1003–13.PubMedCrossRefGoogle Scholar
  3. 3.
    Abramowitz J, Birnbaumer L. Properties of the hormonally responsive rabbit luteal adenylyl cyclase: effects of guanine nucleotides and magnesium ion on stimulation by gonadotropin and catecholamines. Endocrinology 1982; 110:773–81.PubMedCrossRefGoogle Scholar
  4. 4.
    Abramowitz J, Iyengar R, Birnbaumer L. Guanine nucleotide and magnesium ion regulation of the interaction of gonadotropic and beta-adrenergic receptors with their hormones: a comparative study using a single membrane system. Endocrinology 1982; 110:336–46.PubMedCrossRefGoogle Scholar
  5. 5.
    Stormshak F, Casida LE. Effects of LH and ovarian hormones on corpora lutea of pseudopregnant and pregnant rabbits. Endocrinology 1965; 77:337–42.PubMedCrossRefGoogle Scholar
  6. 6.
    Harwood JP, Dufau ML, Catt KJ. Differing specificities in the desensitization of ovarian adenylyl cyclase by epinephrine and human chorionic gonadotropin. Mol Pharmacol 1979; 15:439–44.PubMedGoogle Scholar
  7. 7.
    Catt KJ, Harwood JP, Richert ND, Conn PM, Conti M, Dufau ML. Luteal desensitization: hormonal regulation of LH receptors, adenylate cyclase and steroidogenic responses in the luteal cell. Adv Exp Med Biol 1979; 112:647–62.PubMedCrossRefGoogle Scholar
  8. 8.
    Sibley DR, Lefkowitz RJ. Molecular mechanisms of receptor desensitization using the beta-adrenergic receptor-coupled adenylate cyclase system as a model. Nature 1985; 317:124–9.PubMedCrossRefGoogle Scholar
  9. 9.
    Gilman AG. G proteins: transducers of receptor-generated signals. Annu Rev Biochem 1987;56:615–49.PubMedCrossRefGoogle Scholar
  10. 10.
    Sigafoos JF, Abramowitz J. Comparison of the ability of seven gonadotropin preparations from different mammalian sources to interact with the adenylyl cyclase system in corpora lutea from rabbits and rats. Comp Biochem Physiol 1987; 86A:453–60.CrossRefGoogle Scholar
  11. 11.
    Iyengar R, Abramowitz J, Bordelon-Riser ME, Blume AJ, Bimbaumer L. Regulation of hormone-receptor coupling to adenylyl cyclase. Effects of GTP and GDP. J Biol Chem 1980; 255:10312–21.PubMedGoogle Scholar
  12. 12.
    Sigafoos JF, Abramowitz J. Effects of N-ethylmaleimide on gonadotropin and beta-adrenergic receptor function coupled to rabbit luteal adenylyl cyclase. Endocrinology 1986;119:1432–8.PubMedCrossRefGoogle Scholar
  13. 13.
    Abramowitz J, Bimbaumer L. Temporal characteristics of gonadotropin interaction with rabbit luteal receptors and activation of adenylyl cyclase: comparison to the mode of activation of catecholamine receptors. Endocrinology 1982; 111:970–6.PubMedCrossRefGoogle Scholar
  14. 14.
    Hunzicker-Dunn M, Bimbaumer L. Adenylyl cyclase activities in ovarian tissues. II. Regulation of responsiveness to LH, FSH, and PGEl in the rabbit. Endocrinology 1976; 99:185–97.PubMedCrossRefGoogle Scholar
  15. 15.
    Kirchick JH, Iyengar R, Bimbaumer L. Human chorionic gonadotropin-induced heterologous desensitization of adenylyl cyclase from highly luteinized rat ovaries: attenuation of regulatory N component activity. Endocrinology 1983; 113:1638–46.Google Scholar
  16. 16.
    Briggs MM, Stadel JM, Iyengar I, Lefkowitz RJ. Functional modification of the guanine nucleotide regulatory protein after desensitization of turkey erythrocytes by catecholamines. Arch Biochem Biophys 1983; 142–51.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Bhanu P. Jena
    • 1
  • Joel Abramowitz
    • 1
  1. 1.Department of ZoologyIowa State University of Science and TechnologyAmesUSA

Personalised recommendations