Isolation of Steroidogenic Cell Subpopulations in the Follicular Theca of the Ovary in the Domestic Fowl

  • Enrique Pedernera
  • Pedro Velazquez
  • Yolanda Gomez
  • Margarita Gonzalez del Pliego


The granulosa and the theca of the follicle are the principal source of steroid hormones in the ovary of the domestic fowl. The production of progesterone has been demonstrated in the granulosa layer, while the secretion of 17β-estradiol, testosterone and androstenedione has been found in the theca cells (1–4).


Granulosa Cell Theca Cell Trypsin Treatment Domestic Fowl Preovulatory Follicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Huang ES, Kao KJ, Nalbandov AV. Synthesis of sex steroids by cellular components of chicken follicles. BiolReprod 1979; 20:454–61.CrossRefGoogle Scholar
  2. 2.
    Hammond RW, Todd H, Hertelendy F. Effect of mammalian gonadotropins on progesterone release and cyclic nucleotide production by isolated avian granulosa cells. Gen Comp Endocrinol 1980; 41:467–76.PubMedCrossRefGoogle Scholar
  3. 3.
    Wells JW, Dick, HR., Gilbert AB. The biosynthesis of progesterone by fowl granulosa cells in vitro from C labeled substrates. J Steroid Biochem 1981 ; 14:651–6.Google Scholar
  4. 4.
    Marrone BL, Hertelendy F. Decreased androstenedione production with increased follicular maturation in theca cells from the domestic fowl (Gallus domesticus). J Reprod Fertil 1985; 74:543–50.PubMedCrossRefGoogle Scholar
  5. 5.
    Armstrong DG. Ovarian aromatase activity in the domestic fowl (Gallus domesticus). J Endocrinol 1984; 100:81–6.PubMedCrossRefGoogle Scholar
  6. 6.
    Marrone BL, Hertelendy F. Steroidogenesis by avian ovarian cells: effects of luteinizing hormone and substrate availability. Am J Physiol (Endocrinol Metab 7) 1983; 244:E487–93.Google Scholar
  7. 7.
    Velazquez P, Gomez Y, Gonzalez del Pliego M, Pedernera E. Cambiosen la actividad esteroidogenica de la teca de foliculos preovulatorios de aves [Abstract]. In: Memorias del Congreso Nacional de Ciencias Fisiologicas. Queretaro 1988:95.Google Scholar
  8. 8.
    Pedernera E, Gomez Y, Velazquez P, Juarez-Oropeza MA, Gonzalez del Pliego M. Identification of steroidogenic cell subpopulations in the ovary of the newly hatched chicken. Gen Comp Endocrinol 1988 (in press).Google Scholar
  9. 9.
    Dahl E. Studies of the fine structure of ovarian interstitial tissue 2. The ultrastructure of the thecal gland of the domestic fowl. Z Zellforsch 1970; 109:195–211.PubMedCrossRefGoogle Scholar
  10. 10.
    Gilbert AB, Evans A J, Perry MM, Davidson MH. A method for separating the granulosa cells, the basal lamina and the theca of the preovulatory ovarian follicle of the domestic fowl (Gallus domesticus). J Reprod Fertil 1977; 50:179–81.PubMedCrossRefGoogle Scholar
  11. 11.
    Tennant JR. Evaluation of trypan blue technique for the determination of cell viability. Transplantation 1964; 2:685–94.PubMedCrossRefGoogle Scholar
  12. 12.
    Pedernera E, Gomar Y. Onset of the response to chorionic gonadotropin in the chick embryo testis. Gen Comp Endocrinol 1984; 54:344–9.PubMedCrossRefGoogle Scholar
  13. 13.
    Dahl E. Studies of the fine structure of ovarian interstitial tissue 1. A comparative study of the fine structure of the ovarian interstitial tissue in the rat and the domestic fowl. J Anat 1971; 108:275–90.PubMedGoogle Scholar
  14. 14.
    Porter TE, El Halawani ME. Differential steroidogenesis between theca interna and theca externa layers of preovulatory follicles in the domestic turkey [Abstract]. Biol Reprod 1987; 36(suppl 1):92.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Enrique Pedernera
    • 1
  • Pedro Velazquez
    • 2
  • Yolanda Gomez
    • 1
  • Margarita Gonzalez del Pliego
    • 1
  1. 1.Departamento de Embriologia, Facultad de MedicinaUniversidad Nacional Autonoma de MexicoMexico
  2. 2.Departamento de Histologia, Facultad de MedicinaUniversidad Nacional Autonoma de MexicoMexico

Personalised recommendations