Vasoactive Intestinal Peptide (VIP) Has an LH-Like Action on In Vitro Follicular Steroidogenesis But Not on Ovulation in the Cyclic Hamster

  • Y. Nakamura
  • B. K. Gangrade
  • P. F. Terranova


In vitro Vasoactive Intestinal Peptide (VIP) (but not Substance P) increases ovarian progesterone (P), androstenedione (A) and estradiol (E2) production in the prepubertal rat (1); the source of P and A was interstitium and/or theca. VIP also stimulates in vitro granulosa cell production of P and E2 (2). The VIP-induced E2 secretion was mediated by an increase in granulosa cell aromatase activity (2). These steroidogenic actions of VIP are similar to FSH and LH. The in vitro effects of VIP on steroidogenesis of various types of follicles, i.e., preantral, preovulatory and atretic, are unknown. Thus, our aims were to determine whether VIP altered steroidogenesis in thecae and/or granulosa cells of follicles in various stages of development.


Granulosa Cell Vasoactive Intestinal Peptide Preantral Follicle Atretic Follicle Preovulatory Follicle 
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  1. 1.
    Ahmed CE, Dees WL, Ojeda SR. The immature rat ovary is innervated by vasoactive intestinal peptide (VEP)-containing fibers and responds to VIP with steroid secretion. Endocrinology 1986; 118(4): 1682–9.PubMedCrossRefGoogle Scholar
  2. 2.
    Davoren JB, Hsueh AJW. Vasoactive intestinal peptide: a novel stimulator of steroidogenesis by cultured rat granulosa cells. Biol Reprod 1985; 33:37–52.PubMedCrossRefGoogle Scholar
  3. 3.
    Terranova PF, Martin NC, Chien S. Theca is the source of progesterone in experimentally induced atretic follicles of the hamster. Biol Reprod 1982; 26:721–7.PubMedCrossRefGoogle Scholar
  4. 4.
    Terranova PF. Steroidogenesis in experimentally induced atretic follicles of the hamster: a shift from estradiol to progesterone synthesis. Endocrinology 1981; 108(5): 1885–90.PubMedCrossRefGoogle Scholar
  5. 5.
    Poulin O, Suzuki Y, Lederis K, Rorstad OP. Autoradiographic localization of binding sites for vasoactive intestinal peptide (VIP) in bovine cerebral arteries. Brain Res 1986; 381:382.PubMedCrossRefGoogle Scholar
  6. 6.
    Terranova PF, Garza F. Relationship between the preovulatory luteinizing hormone (LH) surge and androstenedione synthesis of preantral follicles in the cyclic hamster: detection by in vitro responses to LH. Biol Reprod 1983; 29:630–6.PubMedCrossRefGoogle Scholar
  7. 7.
    Burden HW, Lawrence IE Jr, Louis TM, Hadson CA. Effects of abdominal vagotomy on the estrous cycle of the rat and the induction of pseudopregnancy. Neuroendocrinol-ogy 1981; 33:218–22.CrossRefGoogle Scholar
  8. 8.
    Cruz ME, Chavez R, Dominguez R. Ovulation, follicular growth and ovarian reactivity to exogenous gonadotropins in adult rats with unilateral or bilateral section of the vagi nerves. Rev Invest Clin 1986; 38:167–71.PubMedGoogle Scholar
  9. 9.
    Straus E, Keltz TN, Yalow RS. Enzymatic degradation of VIP. In: Said SI, ed. Vasoactive intestinal peptide. New York: Raven Press, 1982:333–9.Google Scholar
  10. 10.
    Johnson AL, Tilly JL. Effects of vasoactive intestinal peptide on steroid secretion and plasminogen activator activity in granulosa cells of the hen. Biol Reprod 1988; 38:296–303.PubMedCrossRefGoogle Scholar
  11. 11.
    Liu YX, Kasson BG, Dahl KD, Hsueh AJW. Vasoactive intestinal peptide stimulates plasminogen activator activity by cultured rat granulosa cells and cumulus-oocyte complexes. Peptides (Fayetteville) 1987; 8:29–33CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Y. Nakamura
    • 1
  • B. K. Gangrade
    • 1
  • P. F. Terranova
    • 1
  1. 1.Department of PhysiologyUniversity of Kansas Medical CenterKansas CityUSA

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