In Vitro Secretion of Angiogenic Activity by Ovine Follicles

  • T. Taraska
  • L. P. Reynolds
  • D. A. Redmer


Earlier investigators noted the tremendous vascularity of ovarian follicles (1–3). This rich blood supply is reflected by the large rate of blood flow to ovarian follicles compared with other tissues (4). During the estrous cycle, growth of vascular beds and increased blood flow accompanies tissue growth of developing follicles (1,2,4), whereas degeneration of follicular blood vessels occurs during atresia (1,5). These observations suggest that an-giogenesis is an important component of follicular function.


Luteinizing Hormone Granulosa Cell Estrous Cycle Follicular Fluid Ovarian Follicle 
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  1. 1.
    Clark JG. The origin, development and degeneration of blood-vessels of the human ovary. John Hopkins Hosp Rep 1900; 9:593–676.Google Scholar
  2. 2.
    Bassett DL. The changes in the vascular pattern of the ovary of the albino rat during the estrous cycle. Am J Anat 1943; 73:251–91.CrossRefGoogle Scholar
  3. 3.
    Burr JH Jr, Davies JI. The vascular system of the rabbit ovary and its relationship to ovulation. Anat Rec 1951; 111:273–92.PubMedCrossRefGoogle Scholar
  4. 4.
    Bruce NW, Moor RM. Capillary blood flow to ovarian follicles, stroma and corpora lutea of anesthesized sheep. J Reprod Fertil 1976; 46:299–304.PubMedCrossRefGoogle Scholar
  5. 5.
    Hay MF, Cran DG, Moor RM. Structural changes occurring during atresia in sheep ovarian follicles. Cell Tissue Res 1976; 169:515–29.PubMedCrossRefGoogle Scholar
  6. 6.
    DiZerega GS, Hodgen GD. Fluorescence localization of luteinizing hormone/human chorionic gonadotropin uptake in the primate ovary. II. Changing distribution during selection of the dominant follicle. J Clin Endocrinol Metab 1980; 51:903–7.PubMedCrossRefGoogle Scholar
  7. 7.
    McNatty KP, Dobson C, Gibb M, Kieboom L, Thurley DC. Accumulation of luteinizing hormone, oestradiol and androstenedione by sheep ovarian follicles in vivo. J Endocrinol 1981; 91:99–109.PubMedCrossRefGoogle Scholar
  8. 8.
    Zeleznik A J, Schuler HM, Reichert LE Jr. Gonadotropin-binding sites in the rhesus monkey ovary: role of the vasculature in the selective distribution of human chorionic gonadotropin to the preovulatory follicle. Endocrinology 1981; 109:356–62.PubMedCrossRefGoogle Scholar
  9. 9.
    Hay MF, Moor RM, Cran DG, Dott HM. Regeneration of atretic sheep ovarian follicles in vitro. J Reprod Fertil 1979; 55:195–207.PubMedCrossRefGoogle Scholar
  10. 10.
    Makris A, Ryan KJ, Yasumizu T, Hill CL, Zetter BR. The nonluteal porcine ovary as a source of angiogenic activity. Endocrinology 1984; 115:1672–7.PubMedCrossRefGoogle Scholar
  11. 11.
    Koos RD, LeMaire WJ. Evidence for an angiogenic factor from rat follicles. In: Greenwald GS, Terranova PF, eds. Factors regulating ovarian function. New York: Raven Press, 1983:191–5.Google Scholar
  12. 12.
    Sato E, Ishibashi T, Koide SS. Inducement of blood vessel formation by ovarian extracts from mice injected with gonadotropins. Experientia 1982; 38:1248–9.PubMedCrossRefGoogle Scholar
  13. 13.
    Rone JD, Goodman AL. Detection of angiotropic activity from intact rabbit follicles cultured in serum-free media [Abstract]. Biol Reprod 1985; 32(suppl 1): 184.Google Scholar
  14. 14.
    Redmer DA, Grazul AT, Kirsch JD, Reynolds LP. Angiogenic activity of bovine corpora lutea at several stages of luteal development. J Reprod Fertil 1988; 82:627–34.PubMedCrossRefGoogle Scholar
  15. 15.
    Reynolds LP, Millaway DS, Kirsch JD, Infeld JE, Redmer DA. Angiogenic activity of placental tissues of cows. J Reprod Fertil 1987; 81:233–40.PubMedCrossRefGoogle Scholar
  16. 16.
    SAS User’s Guide: Statistics. 5th ed. Cary, NC: SAS Institute Inc., 1985.Google Scholar
  17. 17.
    Moor RM, Hay MF, Dott HM, Cran DG. Macroscopic identification and steroidogenic function of atretic follicles in sheep. J Endocrinol 1978; 77:309–18.PubMedCrossRefGoogle Scholar
  18. 18.
    Steel RGD, Torrie JH. Principles and procedures of statistics: a biomedical approach. New York: McGraw-Hill, 1980.Google Scholar
  19. 19.
    Shepro D, D’Amore PA. Physiology and biochemistry of the vascular wall endothelium. In: Renken EM, Michel CC, eds. Handbook of physiology. Bethesda: Am Physiol Soc, 1984:103–64.Google Scholar
  20. 20.
    Kitai H, Yoshimura Y, Wright KH, Santulli R, Wallach EE. Microvasculature of preovulatory follicles: comparison of in situ and in vitro perfused rabbit ovaries following stimulation of ovulation. Am J Obstet Gynecol 1985; 152:889–95.PubMedGoogle Scholar
  21. 21.
    Reynolds SRM. Blood and lymph vascular systems of the ovary. In: Greep RO, Astwood EB,eds. Handbook of physiology. Bethesda: Am Physiol Soc, 1973:261–316.Google Scholar
  22. 22.
    Redmer DA, Rone JD, Goodman AL. Detection of angiotropic activity from primate dominant follicle [Abstract]. 67th Annu Mtg Endocrine Soc 1985:151.Google Scholar
  23. 23.
    Koos RD. Stimulation of endothelial cell proliferation by rat granulosa cell-conditioned medium. Endocrinology 1986; 119:481–9.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • T. Taraska
    • 1
  • L. P. Reynolds
    • 1
  • D. A. Redmer
    • 1
  1. 1.Department of Animal and Range SciencesNorth Dakota State UniversityFargoUSA

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