Gap Junctions in Transformed Rat Granulosa Cells
Granulosa cells perform a variety of functions essential for ovarian cyclicity, ovulation, fertilization and maintenance of pregnancy. Because of their diverse functions and crucial roles in reproduction, granulosa cells are among the most intensively studied endocrine cells (1). However, the properties of granulosa cells in vitro are not necessarily indicative of their properties in vivo because of their marked propensity toward luteinization. Granulosa cells in culture undergo rapid luteinization following treatments with gonadotropins or other agents which stimulate the intracellular formation of cyclic adenosine monophosphate (cAMP) (2), following incubation in media containing serum (3) or even in the absence of known differentiation factors (4,5). The luteinized granulosa cell acquires properties that are quite different from the nonluteinized precursor, losing mitotic and aromatase activities and producing greatly increased amounts of progesterone. Therefore, the properties of luteinized granulosa cells arc in many ways more relevant to functions of corpora lutea than to follicles.
KeywordsGranulosa Cell Cholera Toxin Intercellular Communication Luteinized Granulosa Cell Ovarian Cyclicity
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