Initial Characterization of a Luteal Growth Factor for Ovarian Mesothelial Cells

  • Santo V. Nicosia
  • Beatriz O. Saunders


The ovarian surface epithelium is a modified mesothelial tissue which undergoes species-dependent morphogenetic events throughout life (1,2). The mechanisms regulating such events are poorly understood in spite of the relevance of this tissue in reproduction and cancer (3,4). We have postulated that ovarian mesothelial cells may be influenced by underlying growth signals based on the observation of highly polarized DNA synthesis and morphogenesis in the postovulatory rabbit ovary (5). In the present study, we report on the isolation, biological activity and initial characterization of a recently discovered intraluteal growth factor for rabbit ovarian mesothelial cells (6).


Corpus Luteum Growth Effect Ovarian Surface Epithelium Ovarian Surface Initial Characterization 
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  1. 1.
    Motta PM, Van Blerkom J, Makabe S. Changes in the surface morphology of ovarian germinal epithelium during the reproductive cycle and in some pathological conditions. J Submicrosc Cytol 1980; 12:407–15.Google Scholar
  2. 2.
    Nicosia SV. Morphological changes of the human ovary throughout life. In: Serra GB, ed. The ovary. New York: Raven Press, 1983:57–81.Google Scholar
  3. 3.
    Bjersing L, Cajander S. Ovulation and the mechanism of follicle rupture. III. Transmission electron microscopy of rabbit germinal epithelium prior to induced ovulation. Cell Tissue Res 1974; 149:313–27.PubMedCrossRefGoogle Scholar
  4. 4.
    Nicosia SV, Nicosia RF. Neoplasms of the ovarian mesothelium. In: Azar HA, ed. Pathology of human neoplasms. New York: Raven Press, 1988:435–86.Google Scholar
  5. 5.
    Osterholzer HO, Johnson JH, Nicosia SV. An autoradiographic study of the rabbit ovarian surface epithelium before and after ovulation. Biol Reprod 1985; 33:729–38.PubMedCrossRefGoogle Scholar
  6. 6.
    Nicosia SV, Saunders BO. Corpus luteum extract stimulates the growth of ovarian surface epithelial cells. Lab Invest 1986; 54:47a.Google Scholar
  7. 7.
    Bradford MN. A rapid and sensitive method forthe quantitation of microgram quantities of proteins utilizing a principle of protein-dye binding. Ann Biochem 1976; 72:238–54.CrossRefGoogle Scholar
  8. 8.
    Nicosia SV, Johnson JH, Streibel EJ. Isolation and ultrastructure of rabbit ovarian mesothelium (surface epithelium). Int J Gynecol Pathol 1984; 3:338–60.CrossRefGoogle Scholar
  9. 9.
    Nicosia SV, Johnson JH, Streibel EJ. Growth characteristics of rabbit ovarian mesothelial (surface epithelial) cells. Int J Gynecol Pathol 1985; 4:58–74.PubMedCrossRefGoogle Scholar
  10. 10.
    Goustin AS, Leof EB, Shipley GD, Moses HL. Growth factors and cancer. Cancer Res 1986;46:1015–29.PubMedGoogle Scholar
  11. 11.
    Nicosia SV, Johnson JH. Surface morphology of ovarian mesothelium (surface epithelium) and of other pelvic and extrapelvic mesothelial sites in the rabbit. Int J Gynecol Pathol 1984; 3:249–60.PubMedCrossRefGoogle Scholar
  12. 12.
    Mukku VR, Stancel GM. Regulation of epidermal growth factor receptor by estrogen. J Biol Chem 1985; 260:9820–4.PubMedGoogle Scholar
  13. 13.
    Osterholzer HO, Streibel EJ, Nicosia SV. Growth effects of protein hormones on cultured rabbit ovarian surface epithelial cells. Biol Reprod 1985; 33:247–58.PubMedCrossRefGoogle Scholar
  14. 14.
    Gospodarowicz D, Cheng J, Lui GM, Baird A, Esch F, Bohlen P. Corpus luteum angiogenic factor is related to fibroblast growth factor. Endocrinology 1985; 117:2283–391.CrossRefGoogle Scholar
  15. 15.
    Lobb DK, Kobrin MC, Kudlow JE, Dorrington JH. Transforming growth factor-alpha production by bovine thecal cells. Biol Reprod 1988; 38(1): 191a.Google Scholar
  16. 16.
    Mondschein JS, Hammond JM, Canning SF. Profiles of immunoreactive insulin-like growth factors I and II in porcine follicular fluid and granulosa cell conditioned medium. BiolReprod 1988; 38(l):191a.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Santo V. Nicosia
    • 1
  • Beatriz O. Saunders
    • 1
  1. 1.Department of Pathology, College of MedicineUniversity of South FloridaTampaUSA

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