Abstract
Although germinal vesicle (GV) stage oocytes collected before the gonadotropin surge can resume meiosis relatively easily and reach metaphase II in vitro, the potential for fertilization and embryonic development of these in vitro matured oocytes is usually poor. To understand the role of the gonadotropin surge in controlling the physiological maturation of oocytes, we attempted to mature GV stage mouse oocytes, which had not been exposed to a gonadotropin surge, in medium supplemented by gonadotropins, and then assessed the physiological maturity of the oocytes by examining their potential for fertilization and embryonic development in vitro.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Schroeder AC, Eppig JJ. The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev Biol 1984; 102:493–7.
Whitten WK. Nutrient requirements for the culture of preimplantation embryos in vitro. AdvBiosci 1971; 6:129–41.
Downs SM, Schroeder AC, Eppig J J. Developmental capacity of mouse oocytes following maintenance of meiotic arrest in vitro. Gamete Res 1986; 15:305–16.
Salustri A, Petrungaro S, De Felici M, Conti M, Siracusa G. Effect of follicle-stimulating hormone on cyclic adenosine monophosphate level and on meiotic maturation in mouse cumulus cell-enclosed oocytes cultured in vitro. Biol Reprod 1985; 33:797–802.
Schultz RM, Montgomery RR, Belanoff JR. Regulation of mouse oocyte meiotic maturation: implication of a decrease in oocyte cAMP and protein dephosphorylation in commitment to resume meiosis. Dev Biol 1983; 97:264–73.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Jinno, M., Sandow, B.A., Iizuka, R., Hodgen, G.D. (1989). Gonadotropins Enhance the Cytoplasmic Maturation of Mouse Oocytes Under the Influence of Growth Factors. In: Hirshfield, A.N. (eds) Growth Factors and the Ovary. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5688-2_25
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5688-2_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5690-5
Online ISBN: 978-1-4684-5688-2
eBook Packages: Springer Book Archive