Abstract
The objective of the present study was to determine if the peptides secreted by bovine thecal cells influenced the growth and differentiation of bovine granulosa cells. Bovine thecal cells were cultured under serum-free conditions, the conditioned medium was concentrated and the peptides fractionated on a Bio-Gel P-60 column in 1.0 M acetic acid. Fractions were tested for their ability to stimulate [3H]thymidine incorporation into bovine granulosa cell DNA. Growth-promoting activity was located in the 6,000–9,000 and the 16,000 molecular weight fractions. The peak fractions inhibited FSH-induced aromatase activity in rat granulosa cells, indicating the presence of TGFα-like peptides. A growth inhibitory activity was observed in fractions with a molecular weight of approximately 25,000. The inhibitory activity had the molecular weight of TGFβ, and TGFβ was found to inhibit [3H]thymidine incorporation into bovine granulosa cell DNA. The presence of TGFβ-like activity was confirmed in two independent bioassays; stimulation of FSH-induced aromatase activity, and increased [3H]thymidine incorporation into rat granulosa cell DNA in the presence of FSH. In summary, bovine thecal cells secrete TGFα- and TGFβ-like activities that have opposing actions on the growth of bovine granulosa cells. The relative amounts of these growth factors may determine the rate of growth of granulosa cells during follicular development.
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© 1989 Plenum Press, New York
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Lobb, D.K., Dorrington, J.H. (1989). Bovine Thecal Cells Secrete Transforming Growth Factor α and β. In: Hirshfield, A.N. (eds) Growth Factors and the Ovary. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5688-2_19
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DOI: https://doi.org/10.1007/978-1-4684-5688-2_19
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5690-5
Online ISBN: 978-1-4684-5688-2
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