Insulin-Like Growth Factor-I and Transforming Growth Factor-β Regulate the Differentiation of Purified Ovarian Theca-Interstitial Cells in Serum-Free Medium
The ovarian theca-interstitial cells (TIC) are the source of androgens which are essential for follicle estrogen synthesis (1). Regulation of TIC androgen synthesis is critical for normal follicle development and ovulation. Although luteinizing hormone (LH) is the principal regulator of TIC androgen production (1), recent evidence suggests that intraovarian factors may modulate LH action by autocrine and paracrine mechanisms. Two such factors are insulin-like growth factor-I (IGF-I) secreted by granulosa cells (2) and transforming growth factor-β (TGF-β) produced by TIC (3). Although it has been shown that IGF-I increases LH-stimulated TIC androgen synthesis (4,5), the effects of TGF-β on TIC are unknown. The purpose of these studies was to test the effect of TGF-β on LH and IGF-I stimulated TIC androgen synthesis.
KeywordsLuteinizing Honnone Granulosa Cell Follicle Development Androgen Synthesis Ovarian Androgen
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