Insulin-Like Growth Factor-I and Transforming Growth Factor-β Regulate the Differentiation of Purified Ovarian Theca-Interstitial Cells in Serum-Free Medium

  • Denis A. Magoffin
  • Gregory F. Erickson


The ovarian theca-interstitial cells (TIC) are the source of androgens which are essential for follicle estrogen synthesis (1). Regulation of TIC androgen synthesis is critical for normal follicle development and ovulation. Although luteinizing hormone (LH) is the principal regulator of TIC androgen production (1), recent evidence suggests that intraovarian factors may modulate LH action by autocrine and paracrine mechanisms. Two such factors are insulin-like growth factor-I (IGF-I) secreted by granulosa cells (2) and transforming growth factor-β (TGF-β) produced by TIC (3). Although it has been shown that IGF-I increases LH-stimulated TIC androgen synthesis (4,5), the effects of TGF-β on TIC are unknown. The purpose of these studies was to test the effect of TGF-β on LH and IGF-I stimulated TIC androgen synthesis.


Luteinizing Honnone Granulosa Cell Follicle Development Androgen Synthesis Ovarian Androgen 
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  1. 1.
    Erickson GF, Magoffin DA, Dyer CA, Hofeditz C. The ovarian androgen producing cells: a review of structure/function relationships. Endocr Rev 1985; 6:371–99.PubMedCrossRefGoogle Scholar
  2. 2.
    Hsu C-J, Hammond JM. Gonadotropins and estradiol stimulate immunoreactive insulin-like growth factor-I production by porcine granulosa cells in vitro. Endocrinology 1987; 120:198–207.PubMedCrossRefGoogle Scholar
  3. 3.
    Skinner MK, Keski-Oja J, Osteen KG, Moses HL. Ovarian thecal cells produce transforming growth factor-β which can regulate granulosa cell growth. Endocrinology 1987; 121:786–92.PubMedCrossRefGoogle Scholar
  4. 4.
    Magoffin DA, Erickson GF. An improved method for primary culture of ovarian androgen-producing cells in serum-free medium: effect of lipoproteins, insulin, and insulin-like growth factor-I. In Vitro Cell Dev Biol 1988 (in press).Google Scholar
  5. 5.
    Hernandez ER, Resnick CE, Svoboda ME, Van Wyk JJ, Payne DW, Adashi EY. Somatomedin-C/insulin-like growth factor I as an enhancer of androgen biosynthesis by cultured rat ovarian cells. Endocrinology 1988; 122:1603–22.PubMedCrossRefGoogle Scholar
  6. 6.
    Magoffin DA, Erickson GF. Primary culture of differentiating ovarian androgen-producing cells in defined medium. J Biol Chem 1982; 257:4507–13.PubMedGoogle Scholar
  7. 7.
    Magoffin DA, Erickson GF. Purification of ovarian theca-interstitial cells by density gradient centrifugation. Endocrinology 1988; 122:2345–7.PubMedCrossRefGoogle Scholar
  8. 8.
    DeLean A, Munson PJ, Rodbard D. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Am J Physiol 1978; 235:E97-E102.PubMedGoogle Scholar
  9. 9.
    Sporn MB, Roberts AB, Wakefield LM, deCrombrugghe B. Some recent advances in the chemistry and biology of transforming growth factor-beta. J Cell Biol 1987; 105:1039–45.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Denis A. Magoffin
    • 1
  • Gregory F. Erickson
    • 1
  1. 1.Department of Reproductive Medicine, M-025University of California, San DiegoLa JollaUSA

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