Steroidogenic Effect of Insulin May Not Be Mediated Through Somatomedin-C/IGF-I Receptors

  • A. D. Gooneratne
  • P. A. Thacker
  • B. Laarveld
  • B. D. Murphy
  • K. Rajkumar


Insulin and Somatomedin-C/insulin-like growth factor-I (IGF-I) are capable of eliciting selective differentiative changes in ovarian granulosa cells of several species including growth promotion, enhanced steroidogenesis and induction of luteinizing hormone (LH) receptors (1–3). Insulin and IGF-I can also amplify the stimulatory effects of classical ovarian effector hormones and low-density lipoproteins (LDL) on progesterone (P4) biosynthesis in ovarian cells (3–5). IGF-I receptors have recently been shown to be present, and it is possible that the action of insulin may be mediated through the IGF-I receptors (2).


Growth Hormone Luteinizing Hormone Granulosa Cell Ovarian Granulosa Cell Progesterone Secretion 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Veldhuis JD, Kolp LA, Juchter D, Veldhuis PP, Garmy JC. Mechanisms subserving insulins differentiating actions on progestin biosynthesis by ovarian cells: studies with cultured swine granulosa cells. Endocrinology 1985; 116:651–9.PubMedCrossRefGoogle Scholar
  2. 2.
    Veldhuis JD, Furlanetto RW, Juchter D, Garmy JC, Veldhuis P. Trophic actions of human somatomedin C/insulin-like growth factor 1 on ovarian cells: in vitro studies with swine granulosa cells. Endocrinology 1985; 116:1235–42.PubMedCrossRefGoogle Scholar
  3. 3.
    Maruo T, Hayashi M, Matsuo H, Ueda Y, Morikawa H, Mochizuki M. Comparison of facilitative roles of insulin and insulin-like growth factor-1 in the functional differentiation of granulosa cells; in vitro studies with the porcine model. Acta Endocrinol (Copenh) 1988; 117:230–40.Google Scholar
  4. 4.
    Veldhuis JD, Nestler JE, Straus JF, Gwynne JJ. Insulin regulates low-density lipoprotein metabolism by swine granulosa cells. Endocrinology 1986; 118:2242–53.PubMedCrossRefGoogle Scholar
  5. 5.
    Veldhuis JD, Nestler JE, Strauss JF, Azimi P, Garmy J, Juchter D. The insulin-like growth factor, Somatomedin C, modulates low density lipoprotein metabolism by swine granulosa cells. Endocrinology 1987; 121:340–6.PubMedCrossRefGoogle Scholar
  6. 6.
    Hsu C-J, Hammond JM. Concomitant effects of growth hormone on secretion of insulin like growth factor 1 and progesterone by cultured porcine granulosa cells. Endocrinology 1987; 121:1343–8.PubMedCrossRefGoogle Scholar
  7. 7.
    Rajkumar K, Malinek J, Murphy BD. Effect of lipoproteins and luteotrophins on progesterone accumulation by luteal cells from the pregnant pig. Steroids 1986; 45:119–34.CrossRefGoogle Scholar
  8. 8.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 1951; 193:265–73.PubMedGoogle Scholar
  9. 9.
    Scatchard G. The attraction of proteins for small molecules and ions. Ann NY Acad Sci 1949; 51:660–72.CrossRefGoogle Scholar
  10. 10.
    Hammond JM, Baranao JLS, Skateris D, Knight AB, Romanus JS, Rechler MM. Production of insulin-like growth factors by ovarian granulosa cells. Endocrinology 1985; 117:2553–5.PubMedCrossRefGoogle Scholar
  11. 11.
    Mather JP, Sato GH. Growth of mouse melanoma cells in hormone supplemented serum-free medium. Exp Cell Res 1979; 120:191–5.PubMedCrossRefGoogle Scholar
  12. 12.
    Adashi EY, Resnick CE, Svoboda ME, Van Wyk J. A novel role for somatomedin-C in the cytodifferentiation of the ovarian granulosa cell. Endocrinology 1984; 115:1227–9.PubMedCrossRefGoogle Scholar
  13. 13.
    Hsueh AJW, Adashi EY, Jones PBC, Welsh TH. Hormonal regulation of the differentiation of cultured ovarian granulosa cells. Endocr Rev 1984; 5:76–127.PubMedCrossRefGoogle Scholar
  14. 14.
    Otani T, Maruo T, Mochizuki M. Effect of insulin on porcine granulosa cells: implication of a possible receptor mediated action. Acta Endocrinol (Copenh) 1985; 108:104–10.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • A. D. Gooneratne
    • 1
  • P. A. Thacker
    • 1
  • B. Laarveld
    • 1
  • B. D. Murphy
    • 2
  • K. Rajkumar
    • 2
  1. 1.Department of Animal and Poultry ScienceUniversity of SaskatchewanSaskatoonCanada
  2. 2.Department of Obstetrics and GynecologyUniversity of SaskatchewanSaskatoonCanada

Personalised recommendations