Insulin-Like Growth Factors in Lung Cancer Cell Lines

  • Gabriele Jaques
  • Klaus Havemann


Lung cancer is the most common type of cancer in men all over the world and is the leading cause of cancer mortality in males in more than 35 countries. For females, lung cancer is expected to become more common as the percentage of woman smokers continues to increase. In 1980 the World Health Organisation (WHO) estimated that there will be 660,500 new cases of lung cancer worldwide. It is also expected that lung cancer will surpass gastric cancer as the most common tumor globally by the end of the 1980’s.1,2


Small Cell Carcinoma Lung Cancer Cell Line NSCLC Cell Line Thymidine Uptake Small Cell Lung Cancer Cell 
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  1. 1.
    D. M. Parkin, E. Läarä, and C. S. Muir, Estimates of the worldwide frequency of sixteen major cancers in 1980, Int. J. Cancer 41 (1988 in press).Google Scholar
  2. 2.
    World Health Organization, Female lung cancer increases in developed countries, Weekl. epidem. rec. 61:297 (1986).Google Scholar
  3. 3.
    J. D. Minna, G. A. Higgins, and E. J. Glatsten, Cancer of the lung, in: “Principles and Practice of Oncology,” V. T. de Vita, ed., J. B. Lippincott, Philadelphia (1981).Google Scholar
  4. 4.
    A. F. Greco, “Small cell lung cancer,” Grune and Stratton, New York (1981).Google Scholar
  5. 5.
    G. Bepler, G. Jaques, K. Neumann, G. Aumüller, C. Gropp, and K. Havemann, Establishment, growth properties, and morphological characteristics of permanent human small cell lung cancer cell lines, J. Cancer Res. Clin. Oncol. 113:31 (1987).PubMedCrossRefGoogle Scholar
  6. 6.
    A. F. Gazdar, D. N. Carney, E. K. Russell, H. L. Sims, S. B. Baylin, P. A. Bunn, J. G. Guccion, and J. G. Minna, Establishment of continuous clonable cultures of small cell carcinoma of the lung which have amine-precursor uptake and decarboxylation properties, Cancer Res. 40:3502 (1980).PubMedGoogle Scholar
  7. 7.
    O. S. Pettengill, G. D. Sorenson, D. H. Wurster-Hill, T. J. Curphey, W. W. Noll, C. C. Cate, and L. H. Maurer, Isolation and growth characteristics of continuous cell lines from small cell carcinoma of the lung, Cancer 45:906 (1980).PubMedCrossRefGoogle Scholar
  8. 8.
    J. Bergh, K. Nilsson, R. Ekman, and B. Giovanella, Establishment and characterization of cell lines from human small and large cell carcinomas of the lung, Acta path. microbiol immun. Scand., Sect. A 93:133 (1985).Google Scholar
  9. 9.
    H. Baillie-Johnson, P. R. Twentyman, N. E. Fox, G. A. Walls, P. Workman, J. V. Watson, N. Johnson, J. G. Reeve, and N. M. Bleehen, Establishment and characterization of cell lines from patients with lung cancer (predominantly) small cell carcinoma, Br. J. Cancer 52:495 (1985).PubMedCrossRefGoogle Scholar
  10. 10.
    D. N. Carney, A. F. Gazdar, G. Bepler, J. G. Guccion, P. J. Marangos, T. W. Moody, M. H. G. Zweig, and J. D. Minna, Establishment and identification of small cell lung cancer cell lines having classic and variant features, Cancer Res. 45:2913 (1985).PubMedGoogle Scholar
  11. 11.
    P. L. Kaplan, and B. Ozaune, Cellular responsiveness to growth factors correlates with a cell’s ability to express the transformed phenotype, Cell 33:931 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    F. U. Cuttitta, D. N. Carney, J. Mulshine, T. W. Moody, J. Fedorko, A. Fischler, and J. D. Minna, Bombesin-like peptides can function as autocrine growth factors in human small cell lung cancer, Nature 316:823 (1985).PubMedCrossRefGoogle Scholar
  13. 13.
    A. J. D’Ercole, A. D. Stiles, and L. E. Underwood, Tissue concentration of somatomedin-C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanism of action, Proc. Natl. Acad. Sci. USA 81:935 (1984).PubMedCrossRefGoogle Scholar
  14. 14.
    F. Minuto, P. Del Monte, A. Barreca, P. Fortini, G. Cariola, G. Catrambone, and G. Giordano, Evidence for an increased somatomedin-C/insulin-like growth factor I content in primary human lung tumors, Cancer Res. 46:985 (1986).PubMedGoogle Scholar
  15. 15.
    G. Jaques, M. Rotsch, C. Wegmann, U. Worsch, M. Maasberg, and K. Havemann, Production of immunoreactive insulin-like growth factor I and response to exogenous IGF-I in small cell lung cancer cell lines, Exp. Cell Res. 176:336 (1988).PubMedCrossRefGoogle Scholar
  16. 16.
    V. M. Macauly, J. D. Teale, M. J. Everard, G. P. Joshi, I. E. Smith, and J. L. Millar, Somatomedin-C/insulin-like growth factor-I is a mitogen for human small cell lung cancer, Br. J. Cancer 57:91 (1988).PubMedCrossRefGoogle Scholar
  17. 17.
    C. Betsholtz, J. Bergh, M. Bywater, M. Petterson, A. Johnson, C.-H. Heldin, R. Ohlsson, T. J. Knott, J. Scott, G. I. Bell, and B. Westermark, Expression of multiple growth factors in a human lung cancer cell line, Int. J. Cancer 39:502 (1987).PubMedCrossRefGoogle Scholar
  18. 18.
    G. I. Bell, J. P. Merryweather, R. Sanchez-Pescator, M. M. Stempien, L. Preistly, J. Scott, and L. B. Rail, Sequence of a cDNA clone encoding human preproinsulin-like growth factor II, Nature 310:775 (1984).PubMedCrossRefGoogle Scholar
  19. 19.
    D. Yee, R. E. Favoni, K. K. Huff, S. Paik, R. B. Dickson, G. S. Lebovic, A. Schwartz, M. E. Lippman, and N. Rosen, Insulin-like growth factor I (IGF-I) expression and a novel IGF-I related activity in human malignancy, Proc. Am. Assoc. Cancer Res. 29:54 (1988).Google Scholar
  20. 20.
    F. Cuttitta, M. L. Levitt, J. G. Park, P. Kasprzyk, Y. Nakanishi, J. Reeve, J. Walsh, J. Mulshine, A. F. Gazdar, and J. D. Minna, Growth of human cancer cell lines in unsupplemented basal media as a means of identifying autocrine growth factors, Proc. Am. Assoc. Cancer Res. 28:27 (1987).Google Scholar
  21. 21.
    W. H. Daughaday, I. K. Mariz, and S. L. Blethen, Inhibition of bound somatomedin to membrane receptor and immunobinding sites: a comparison of radioreceptor and radioimmunoassay of somatomedin in native and acid-ethanol-extracted serum, J. Clin Endocrinol. Metab. 51:781 (1980).PubMedCrossRefGoogle Scholar
  22. 22.
    T. Minuto, P. Del Monte, A. Barreca, A. Alama, G. Cariola, and G. Giordano, Evidence for autocrine mitogenic stimulation by somatomedin C/insulin-like growth factor I on an establish human lung cancer cell line, Cancer Res. 48:3716 (1988).PubMedGoogle Scholar
  23. 23.
    J. M. Horner, F. Liu, and Hintz, Comparison of [125I] somatomedin A and [I J somatomedin C radioreceptor assays for somatomedin peptide content in whole and acid chromato-graphed plasma, J. Clin. Endocrinol. Metab. 47:1287 (1978).PubMedCrossRefGoogle Scholar
  24. 24.
    M. Ogasawara, K. P. Karey, and D. A. Sirbasku, Insulin-like growth factor I: relationship between plasma and tissue sources, biological potency and N α truncation, Proc. Am. Assoc. Cancer Res. 29:52 (1988).Google Scholar
  25. 25.
    G. Jaques, and K. Havemann, IGF-binding protein in small cell lung cancer cell lines, Exp. Cell Res. (1988 submitted).Google Scholar
  26. 26.
    C. D. Scott, J. L. Martin, and R. C. Baxter, Production of insulin-like growth factor I and its binding protein by adult rat hepatocytes in primary culture, Endocrinology 116:1094 (1985).PubMedCrossRefGoogle Scholar
  27. 27.
    M. A. De Vroede, L. Y. H. Tseng, G. Pamayotis, S. Katsoyannis, S. P. Nissley, and M. M. Rechler, J. Clin. Invest. 77:602 (1986).PubMedCrossRefGoogle Scholar
  28. 28.
    A. J. D’Ercole, and J. R. Wilkins, Affinity labeled somatomedin C binding proteins in rat sera, Endocrinology 114:1141 (1984).PubMedCrossRefGoogle Scholar
  29. 29.
    D. R. Clemmons, R. G. Elgin, V. K. M. Han, S. J. Casella, A. J. D’Ercole, and J. J. Van Wyk, Cultured fibroblast mono-lyers secrete a protein that alters the cellular binding of somatomedin C-insulin-like growth factor I, J. Clin. Invest. 77:1548 (1986).PubMedCrossRefGoogle Scholar
  30. 30.
    O. Ritvos, T. Ranta, J. Jalkanen, A. H. Suikkari, R. Voutilainen, H. Bohn, and E. M. Rutanen, Insulin-like growth factor (IGF) binding protein from human decidua inhibits the binding and biological action of IGF-I in cultured choriocarcinoma cells, Endocrinology 122:2150 (1988)PubMedCrossRefGoogle Scholar
  31. 31.
    R. G. Elgin, W. H. Busby, and D. R. Clemmons, An insulin-like growth factor (IGF) binding protein enhances the biologic response to IGF-I, Proc. Natl. Acad. Sci. 84:3254 (1987).PubMedCrossRefGoogle Scholar
  32. 32.
    P. S. Nissley, and M. M. Rechler, Insulin-like growth factors: biosynthe is, receptors, and carrier proteins, in: “Hormonal proteins and peptides,” Li Ch., ed., Academic Press, Orlando (1984).Google Scholar
  33. 33.
    M. Rotsch, U. Worsen, G. Jaques, M. Maasberg, and K. Havemann, Insulin-like growth factor I binding sites in non small cell lung cancer cell lines, J. Cancer Res. Clin. Oncol. 114:542 (1988).Google Scholar
  34. 34.
    W. D. Salmon, Jr., and M. R. DuVall, A serum fraction with “sulfation factor activity” stimulates in vitro incorporation of leucine and sulfate into protein-polysaccharide complexes, uridine into RNA and thymidine into DNA of costal cartilage from hypophysectomized rats, Endocrinology 86:721 (1970).PubMedCrossRefGoogle Scholar
  35. 35.
    R. C. Baxter, S. Axiak, and R. L. Raison, Monoclonal antibody against human somatomedin-C/insulin-like growth factor I, J. Clin Endocrinol. Metab. 54:474 (1980).CrossRefGoogle Scholar
  36. 36.
    Y. Nakanishi, F. Cuttitta, P. Kasprzyk, I. Avis, S. Steinberg, A. Gazdar, and J. Mulshine, Valiation of a rapid colori-metric assay demonstrating growth stimulatory effects including insulin-like growth factor-I (IGF-I) on small cell lung cancer cell lines (SCLC-CL), Proc. Am. Assoc. Cancer Res. 28:53 (1987).Google Scholar
  37. 37.
    G. Jaques, C. Wegmann, M. Rotsch, M. Maasberg, and K. Havemann, Production and secretion of immunreactive insulin-like growth factor I, J. Cancer Res. Clin. Oncol. 114:S154 (1988).Google Scholar
  38. 38.
    M. B. Sporn, and G. J. Todaro, Autocrine secretion and malignant transformation of cells, N. Engl. J. Med. 303:878 (1980).PubMedCrossRefGoogle Scholar
  39. 39.
    S. M. Wood, J. R. Wood, M. A. Glatei, Y. C. Lee, D. O’Shanghnessy, and S. R. Bloom, Bombesin, somatostatin and neuro-tensin-like immunreactivity in bronchial carcinoma, J. Clin. Endocrinol. Metab. 53:1310 (1981).PubMedCrossRefGoogle Scholar
  40. 40.
    W. Luster, C. Gropp, H. F. Kern, and K. Havemann, Lung tumour cell lines synthesizing peptide hormones established from tumours of four histological types: characterization of the cell lines and analysis of their peptrole hormone production, Br. J. Cancer 51:865 (1985).PubMedCrossRefGoogle Scholar
  41. 41.
    T. W. Moody, C. P. Pert, A. F. Gazdar, D. N. Carney, and J. D. Minna, High levels of intracellular bombesin characterize human small cell lung carcinoma, Science 214:1246 (1981).PubMedCrossRefGoogle Scholar
  42. 42.
    G. D. Sorenson, S. R. Bloom, M. A. Ghateis, S. A. Del Prete, C. C. Cate, and O. S. Pettengill, Bombesin production by human small cell carcinoma of the lung, Regul. Pept. 4:59 (1982).PubMedCrossRefGoogle Scholar
  43. 43.
    T. W. Moody, V. Bertness, and D. N. Carney, Bombesin-like peptides and receptors in human tumor cell lines, Peptides 4:683 (1983).PubMedCrossRefGoogle Scholar
  44. 44.
    S. Weber, J. E. Zuckermann, D. G. Bostwick, K. G. Bensch, B. I. Sikic, and T. A. Raffin, Gastrin releasing peptide is a selective mitogen for small cell lung carcinoma in vitro, J. Clin. Invest. 75:306 (1985).PubMedCrossRefGoogle Scholar
  45. 45.
    D. N. Carney, F. Cuttitta, T. W. Moody, and J. D. Minna, Selective stimulation of small cell lung cancer clonal growth by bombesin and gastrin-releasing peptide, Cancer Res. 47:821 (1987).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Gabriele Jaques
    • 1
  • Klaus Havemann
    • 1
  1. 1.Dept. Internal Medicine, Div. Hematology/OncologyPhilipps-University of MarburgMarburgGermany

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