Regulation of the Neuronal Insulin-Like Peptide

  • S. Devaskar
  • H. F. Sadiq


Insulin or an insulin-like peptide has been demonstrated within the brains of various animal species1 and more importantly insulin immunoreactivity has been observed within a few neurons.2,3 In an attempt to determine whether this peptide is synthesized locally within the CNS, we have previously employed the technique of in-situ hybridization and a biotinylated rat insulin I cDNA to detect insulin mRNA within isolated and cultured 10 day old rabbit neurons. Neurons were characterized and detected by the neuron-specific enolase staining and glial cells contaminating these cultures were detected by the glial fibrillary acidic protein staining. We observed the presence of insulin transcripts within 3 to 5% of neurons (75% pure cultures) that were maintained in a serum-containing medium (Figure 1a). Interestingly, the same percentage of cells was positive for insulin immunoreactivity. Under similar hybridization conditions utilized for insulin, IGF I and II cDNAs (human) failed to cross-hybridize with these “insulin” transcripts.3


Exogenous Insulin Insulin Immunoreactivity Rabbit Brain Insulin Synthesis Glial Fibrillary Acidic Protein Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D. LeRoith, S. A. Hendricks, M. A. Lesniak, S. Rishi, K. L Becker, J. Havrankova, J. L. Rosenweig, M. J. Brownstein and J. Roth, Insulin in brain and other extrapancreatic tissues of vertebrates and non-vertebrates, Adv. Metab. Disorders 10:303–340 (1983).Google Scholar
  2. 2.
    M. K. Raizada, Localization of insulin-like immunoreactivity in the neurons from primary cultures of rat brain, Exp. Cell Res. 143:351–357 (1983).PubMedCrossRefGoogle Scholar
  3. 3.
    R. Schechter, L. Holtzclaw, F. Sadiq, A. Kahn and S. Devaskar, Insulin synthesis by isolated rabbit neurons, Endocrinology 123:505–513 (1988).PubMedCrossRefGoogle Scholar
  4. 4.
    J. E. Bottenstein, Growth and differentiation of neural cells in defined media, in: “Cell Culture in the Neurosciences” (eds. J. E. Bottenstein and G. Sato), Plenum Press, New York (1985).CrossRefGoogle Scholar
  5. 5.
    K. Rau, L. Muglia and J. Locker, Insulin-gene expression in extrafetal membranes of rats, Diabetes 38:39–43 (1989).PubMedCrossRefGoogle Scholar
  6. 6.
    A. Efstratiadis, Unpublished data presented at the Symposium on Insulin, IGFs and Their Receptors, University of Florida College of Medicine, Gainesville, FL, January, 1989.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • S. Devaskar
    • 1
    • 2
  • H. F. Sadiq
    • 1
    • 2
  1. 1.Division of Neonatology, Department of PediatricsSt. Louis UniversitySt. LouisUSA
  2. 2.The Pediatric Research InstituteCardinal Glennon Children’s HospitalSt. LouisUSA

Personalised recommendations