Conditional-Lethal Mutant of Calmodulin in Yeast
Molecular biological and genetic analyses have proved useful in studies on the roles of calmodulin in cell proliferation. Calmodulin genes or its cDNA were isolated from a number of eukaryotes such as electric eel, chicken, human, rat, Xenopus, Dictyostelium, C. elegance and yeasts. Among them, yeasts are the most suitable organisms for genetic analysis. Like E. coli, any gene in yeasts can be replaced with its mutational allele that is constructed in vitro, if its gene has been cloned. Essential gene can also be analyzed by using combination of the genereplacement in a diploid strain and dissecting its tetrad.
KeywordsGlucose Medium Nuclear Division Diploid Strain GALl Promoter Yeast Cell Cycle
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