Calspermin is a Testis Specific Calmodulin-Binding Protein Closely Related to Ca2+/Calmodulin-Dependent Protein Kinases

  • Tomio Ono
  • Anthony R. Means
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 255)


Calmodulin regulates a wide variety of cellular functions by interacting with its binding proteins or enzymes 1. Based on studies with a number of naturally occuring peptides that bind calmodulin with high affinity, it has been predicted that both ionic and hydrophobic interactions are involved in the association and that a general characteristic of a calmodulin-binding peptide is the ability to form a basic, amphiphilic helix 2–5. Indeed as the sequence of calmodulin-binding proteins have become known, the basic, amphiphilic helix has been shown to be the one common feature of the calmodulin-binding domain 6–16.


Deduce Amino Acid Sequence Round Spermatid Nontranslated Region Tissue Specific Alternative Splice Muscle Myosin Light Chain Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    A.R. Means, Rec., Prog. Horm. Res. 44:223 (1988).Google Scholar
  2. 2.
    J.A. Cox, M. Comte, J.E. Fitton, and W.F. DeGrado, J. Biol. Chem. 260:2527 (1985).PubMedGoogle Scholar
  3. 3.
    L. McDowell, G. Sanyal, and F.G. Prendergast, Biochemistry 24:2979 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    D.P. Geidroc, T.M. Keravis, J.V. Staros, N. Ling, J.N. Wells, and D. Puett, Biochemistry 24:1203 (1985).CrossRefGoogle Scholar
  5. 5.
    D.A. Malencik, and S.R. Anderson, Biochem. Biophys. Res. Commun. 114:50 (1983).PubMedCrossRefGoogle Scholar
  6. 6.
    W.F. DeGrado, S. Erickson-Viitanen, H.R. Wolfe, Jr. and K.T. O’Neil, Proteins 2:20 (1987).PubMedCrossRefGoogle Scholar
  7. 7.
    D.K. Blumenthal, K. Takio, A.M. Edelman, H. Charbonneau, K. Titani, K.A. Walsh, and E.G. Krebs, Proc. Natl. Acad. Sci. USA 82:3187 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    V. Guerriero, Jr., M.A. Russo, N.J. Olson, J.A. Putkey, and A.R. Means, Biochemistry 25:8372 (1986).PubMedCrossRefGoogle Scholar
  9. 9.
    T.J. Lukas, W.H. Burgess, F.G. Prendergast, W. Lau, and D.M. Watterson, Biochemistry 25:1458 (1986).PubMedCrossRefGoogle Scholar
  10. 10.
    R.M. Hanley, A.R. Means, T. Ono, B.E. Kemp, K.E. Burgin, N. Waxham, and P.T. Kelly, Science 237:293 (1987).PubMedCrossRefGoogle Scholar
  11. 11.
    C.R. Lin, M.S. Kapiloff, S. Durgerian, K. Tatemoto, A.F. Russo, P. Hanson, H. Schulman, and M.G. Rosenfeld, Proc. Natl. Acad. Sci. USA 84:5962 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    M.K. Bennett, and M.B. Kennedy, Proc. Natl. Acad. Sci. USA 84:1794 (1987).PubMedCrossRefGoogle Scholar
  13. 13.
    B. Buschmeier, H.E. Meyer, and G.W. Mayr, J. Biol. Chem. 262:9454 (1987).PubMedGoogle Scholar
  14. 14.
    P. James, M. Maeda, R. Fischer, A.K. Verma, J. Krebs, J.T. Penniston and E. Carafoli, J. Biol. Chem. 263:2905 (1988).PubMedGoogle Scholar
  15. 15.
    G.E. Shull, and J. Greeb, J. Biol. Chem. 263:8646 (1988).PubMedGoogle Scholar
  16. 16.
    K.A. Alexander, B.T. Wakim, G.S. Doyle, K.A. Walsh, and D.R. Storm, J. Biol. Chem. 263:7544 (1988).PubMedGoogle Scholar
  17. 17.
    T. Ono, Y. Koide, Y. Arai, and K. Yamashita, J. Biol. Chem. 259:9011 (1984).PubMedGoogle Scholar
  18. 18.
    T. Ono, Y. Koide, Y. Arai and K. Yamashita, J. Biochem. (Tokyo) 98:1455 (1985).PubMedGoogle Scholar
  19. 19.
    Y. Koide, T. Ono, and K. Yamashita, Cell Calcium 7:329 (1986).PubMedCrossRefGoogle Scholar
  20. 20.
    T. Ono, Y. Koide, Y. Arai and K. Yamashita, Arch. Biochem. Biophys. 255:102 (1987).PubMedCrossRefGoogle Scholar
  21. 21.
    Y. Koide, T. Ono, C. Ishinami, and K. Yamashita, Amer. J. Physiol. 250:C299 (1986).Google Scholar
  22. 22.
    J.M. Sikela, and W.E. Hahn, Proc. Natl. Acad. Sci. USA 84:3038 (1987).PubMedCrossRefGoogle Scholar
  23. 23.
    J. Kyte, and R.F. Doolittle, J. Mol. Biol. 157:105 (1982).PubMedCrossRefGoogle Scholar
  24. 24.
    J. Gamier, D.J. Osquthorpe and B. Robson, J. Mol. Biol. 120:97 (1978).CrossRefGoogle Scholar
  25. 25.
    S.K. Hanks, A.M. Quinn and T. Hunter, Science 241:42 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Tomio Ono
    • 1
  • Anthony R. Means
    • 1
  1. 1.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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