Analysis of Calcium-Binding Sites in Calcium-Activated Neutral Protease

  • Koichi Suzuki
  • Yasufumi Minami
  • Yasufumi Emori
  • Shinobu Imajoh
  • Hiroshi Kawasaki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 255)


Calcium-activated neutral protease (CANP) is a typical intracellular protease responsible for the processing and turnover of various intracellular proteins.1–5 Uncontrolled, CANP can destroy various cellular components, leading to various disease processes like muscular dystrophy. Hence the activity must be tightly regulated. Because CANP absolutely requires calcium for activity, calcium plays a pivotal role in its regulation. Although many of the important steps of CANP activity regulation have been clarified, most studies have not provided any insight into the binding of calcium to CANP6. Structural analyses of CANP have revealed the existence of a calmodulin-like domain in the C-terminal region of each of the two constituent, subunits (80K and 30K subunits).7–9 These domains are presumed to bind calcium and determine the calcium sensitivity of CANP. Nevertheless, the following questions remain unsolved: whether the calmodulin domains indeed bind calcium; whether calcium-binding sites exist only in the calmodulin domains; and, if so, how many calcium ions bind to each domain and which EF hand structures in the calmodulin domains are functional calcium-binding sites.


Calcium Binding Regulatory Light Chain Double Reciprocal Plot Calcium Binding Site Calcium Coordination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Koichi Suzuki
    • 1
  • Yasufumi Minami
    • 1
  • Yasufumi Emori
    • 1
  • Shinobu Imajoh
    • 1
  • Hiroshi Kawasaki
    • 1
  1. 1.Department of Molecular BiologyTokoyo Metropolitan Institute of Medical ScienceHonkomagome, Bunkyo-ku Tokyo-113Japan

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