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Sarcolemmal (Ca2+ + Mg2+)-ATPase of Vascular Smooth Muscle

  • Shoichi Imai
  • Yutaka Yoshida
  • Hsiao-Tung Sun
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 255)

Abstract

The existence of a plasmalemmal ATP-dependent Ca2+-pump that extrudes Ca2+ out of the smooth muscle cells against its electrochemical gradient is well established and has been studied by measuring the ATP-dependent accumulation of Ca by microsomal fractions of smooth muscles.

Keywords

ATPase Activity Vascular Smooth Muscle Malachite Green Microsome Fraction Dependent Protein Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chan, K.-M., Delfert, D. and Junger, K.D., 1986, A direct colorimetric assay for Ca2+-stimulated ATPase activity, Anal. Biochem., 157:375.PubMedCrossRefGoogle Scholar
  2. Deshutter, G., Wuytack F. and Casteels, R., 1984, Tissue levels and purification by affinity chromatography of the calmodlin-stimulated Ca2+-transport ATPase in pig antrum smooth muscle, Biochim. Biophys. Acta, 773:1.CrossRefGoogle Scholar
  3. Furukawa, K. and Nakamura, H., 1984, Characterization of the (Ca2+ + Mg2+)ATPase purified by calmodulin-affinity chromatography from bovine aortic smooth muscle, J. Biochem., 96:1343.PubMedGoogle Scholar
  4. Furukawa, K. and Nakamura, H., 1987, Cyclic GMP regulation of the plasma membrane (Ca2+-Mg2+) ATPase in vascular smooth muscle, J. Biochem., 101:287.PubMedGoogle Scholar
  5. Jones, L. R. and Besch, H. R. Jr., 1984, Isolation of canine cardiac sarcolemmal vesicles, Methods Pharmacol., 5:1.Google Scholar
  6. Kikkawa, U., Go, M., Koumoto, J. and Nishizuka, Y., 1986, Rapid purification of protein kinase C by high performance liquid chromatography, Biochem. Biophys. Res. Commun., 135:636.PubMedCrossRefGoogle Scholar
  7. Lincoln, T.M., 1983, cGMP-dependent protein kinase, Methods Enzymol., 99:62.PubMedCrossRefGoogle Scholar
  8. Popescu, L. M., Panoiu, C., Hinescu, M. and Nutu, O., The mechanism of cGMP-induced relaxation in vascular smooth muscle, Eur. J. Pharmacol., 107:393Google Scholar
  9. Raeymarkers, L., Wuytack, F. and Casteels, R.’ 1985, Subcellular fractionation of pig stomach smooth muscle. A study of the distribution of the (Ca2++Mg2+)-ATPase activity in plasmalemma and endoplasmic reticulum, Biochim. Biophys. Acta, 815:441.CrossRefGoogle Scholar
  10. Rashatwar, S. S., Cornwell, T. L. and Lincoln, T. M., 1987, Effects of 8-bromo-cGMP on Ca2+ levels in vascular smooth muscle cells: Possible regulation of Ca2+-ATPase by cGMP-dependent protein kinase, Proc. Natl. Acad. Sci., 84:5685.PubMedCrossRefGoogle Scholar
  11. Smallwood, J. I., Giigi, B. and Rasmussen, H., 1988, Regulation of erythrocyte Ca2+ pump activity by protein kinase C, J. Biol. Chem., 263:2195.PubMedGoogle Scholar
  12. Suematu, E., Hirata, M. and Kuriyama, H., 1984, Effects of cAMP-and cGMP-dependent protein kinase, and calmodulin on Ca2+ uptake by highly purified sarcolemmal vesicles of vascular smooth muscle, Biochim. Biophys. Acta, 773:83.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Shoichi Imai
    • 1
  • Yutaka Yoshida
    • 1
  • Hsiao-Tung Sun
    • 1
  1. 1.Department of PharmacologyNiigata University School of MedicineNiigataJapan

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