Transfer of Human HPRT Gene Sequence into Neuronal Cells by a Herpes Simplex Virus Derived Vector

  • T. D. Palella
  • L. J. Silverman
  • F. L. Homa
  • M. Levine
  • W. N. Kelley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)


The purine salvage enzyme, hypoxanthine-guanine phosphoribosyltrans-ferase (HPRT), catalyzes the conversion of hypoxanthine and guanine to the mononucleotides, IMP and GMP, respectively. Complete deficiency of HPRT activity in humans causes the Lesch-Nyhan syndrome, a devastating disease characterized by overproduction of uric acid and severe neurological dysfunction.1, 2 For a variety of reasons, attention has focused on the Lesch-Nyhan syndrome as one of the initial candidates for therapy by somatic cell gene transfer.3


Thymidine Kinase HPRT Activity Human HPRT APRT Gene Herpes Virus Thymidine Kinase 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • T. D. Palella
    • 1
  • L. J. Silverman
    • 1
  • F. L. Homa
    • 1
  • M. Levine
    • 1
  • W. N. Kelley
    • 1
  1. 1.Departments of Internal Medicine, Human Genetics and Biological ChemistryUniversity of MichiganAnn ArborUSA

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