Analysis of Molecular Structure of Rat Phosphoribosylpyrophosphate Synthetase Genes
Phosphoribosylpyrophosphate (PRPP) synthetase catalyzes the formation of PRPP by a pyrophosphoryl transfer from ATP to ribose 5-phosphate. The enzyme has been purified from bacteria [1, 2] and mammalian tissues [3, 4], The mammalian enzyme exists in many active forms of various molecular mass , reportedly composed of subunits of 31, 000–40, 500 daltons [1–4], However, our recent results showed that rat liver PRPP synthetase exists as complex aggregates of 34-kDa, 38-kDa, and 40-kDa components and the 34-kDa species is the catalytic subunit (K. Kita et al., this volume). We then carried out cDNA cloning of rat enzyme  in order to determine the primary structure and also to proceed to studies of gene regulation. We here describe unpublished details of part of the cloning experiments and preliminary accounts of analysis of rat genomic genes.
KeywordsSodium Dodecyl Sulfate Tryptic Peptide Phage Vector Southern Blot Technique PRPP Synthetase
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