Sequence Analysis of Rat Liver Uricase-cDNA and the Possible Presence of the Homologous cDNA Sequences in Chicken Embryo

  • Masaki Ito
  • Masamichi Nakamura
  • Hisamitsu Ogawa
  • Yasuyuki Takagi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)

Abstract

The final product of purine nucleotide metabolism varies from uric acid to ammonia, depending on the species, and it is supposed that the enzymes responsible for the degradation of uric acid have been lost one by one during evolution (1). The loss of the enzymes could be due to the inactivation of the genes. Therefore, in order to understand the mechanism of the gene inactivation, we tried to isolate cDNAs for uricase in various species and compare their structures.

Keywords

Urea Agarose Polypeptide Nylon Pyrimidine 

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References

  1. 1.
    T. Noguchi, Y. Takada and S. Fujiwara, Degradation of uric acid to urea and glyoxylate in peroxisomes, J. Biol. Chem. 254: 5272 (1979)PubMedGoogle Scholar
  2. 2.
    M. Ito, M. Suzuki and Y. Takagi, Nucleotide sequence of cDNA and predicted amino acid sequence of rat liver uricase, Eur. J. Biochem. 173: 459 (1988)PubMedCrossRefGoogle Scholar
  3. 3.
    T. Nguyen, M. Zelechowska, V. Foster, H. Bergmann and D.P.S. Verma, Primary structure of the soybean nodulin-35 gene encoding uricase II localized in the peroxisomes of uninfected cells of nodules, Proc. Natl Acad. Sci. USA 82: 5040 (1985)PubMedCrossRefGoogle Scholar
  4. 4.
    St. J. Przylecki and L. Rogalski, La loi biogenetique et les fonctions des organismes vivants: La presence de l’uricase chez les embryons des oiseaux, Arch. Intern. Physiol. 28: 423 (1927)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Masaki Ito
    • 1
  • Masamichi Nakamura
    • 1
  • Hisamitsu Ogawa
    • 1
  • Yasuyuki Takagi
    • 1
  1. 1.Division of Molecular Genetics, Institute for Comprehensive Medical ScienceFujita-Gakuen Health UniversityToyoake, AichiJapan

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