Effect of Alterations of the ATG Translation Start Codon of the APRT Gene

  • Howard V. Hershey
  • Milton W. Taylor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)


The aprt gene locus has distinct advantages as a model for analysis of mutations in eucaryotic cells in culture. It is, for a eucaryotic gene, quite small. The minimal upstream through downstream region needed for normal function of the CHO gene when transfected into a host has been determined by deletion mapping to be from about 90 nt upstream of the transcription initiation site (about 155 nt upstream of the translation initiation site) through the gene to somewhere between 246 and 430 nt downstream of the translation stop codon. The 3′ untranslated sequence must include the first polyadenylation signal and a signal at least 10 nt and less than 200 nt 3′ of the polyadenylation signal. Thus the functional gene, including introns and 5′ and 3′ signals is somewhere between 2560 and 2380 nt long 1.


Start Codon Chinese Hamster Ovary Cell Initiation Codon Polyadenylation Signal Translation Initiation Site 
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  1. 1.
    J-H. Park and M.W. Taylor, 1988, Analysis of Signals Controlling Expression of the Chinese Hamster Ovary aprt Gene, Mol. Cell. Biol. 8: 2536.PubMedGoogle Scholar
  2. 2.
    A.E. Simon, M.W. Taylor, W.E.C. Bradley, and L. Thompson, 1982, A Model Involving Gene Inactivation in the Generation of Autosomal Recessive Mutants in Mammalian Cells in Culture, Mol. Cell. Biol. 2: 1126.PubMedGoogle Scholar
  3. 3.
    A.E. Simon and M.W. Taylor, 1983, High Frequency Mutation at the Adenine Phosphoribosyltransferase Locus in Chinese Hamster Ovary Cells due to Deletion of the Gene, Proc. Natl. Acad. Sci. USA 80: 810.PubMedCrossRefGoogle Scholar
  4. 4.
    J.O. Nalbantoglu, O. Goncalves, and M. Meuth, 1983, Structure of Mutant Alleles at the aprt Locus of Chinese Hamster Ovary Cells, J. Mol. Biol. 167: 575.PubMedCrossRefGoogle Scholar
  5. 5.
    W.E.C. Bradley, A. Belouchi, and K. Messing, 1988, The aprt Heterozygote/Hemizygote System for Screening Mutagenic Agents Allows Detection of Large Deletions, Mut. Res. 199: 131.CrossRefGoogle Scholar
  6. 6.
    A.J. Grosovsky, E.A. Drobetsky, P.J. de Jong, and B.W. Glickman, 1986, Southern Analysis of Genoraic Alterations in Gamma-Ray-Induced APRT- Hamster Cell Mutants, Genetics 113: 405.PubMedGoogle Scholar
  7. 7.
    L.H. Breimer, J. Nalbantoglu, and M. Meuth, 1986, Structure and Sequence of Mutations Induced by Ionizing Radiation of Selectable Loci in Chinese Hamster Ovary Cells, J. Mol. Biol. 192: 669.PubMedCrossRefGoogle Scholar
  8. 8.
    S.P. Becerra, M. Hardy, B.M. Baroudy, and C.W. Anderson, 1985, Direct Mapping of Adeno-associated Virus Capsid Proteins B and C: A Possible ACG Initiation Codon, Proc. Natl. Acad. Sci. USA 82: 7919.PubMedCrossRefGoogle Scholar
  9. 9.
    J. Nalbantoglu, G.A. Phear, and M. Meuth, 1986, Nucleotide Sequence of Hamster Adenine Phosphoribosyltransferase (aprt) gene, Nucl. Acids Res. 14: 1914.PubMedCrossRefGoogle Scholar
  10. 10.
    T.A. Kunkel, J.D. Roberts, and R.A. Zakour, 1987, Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection, Method. Enzymol. 154: 367.CrossRefGoogle Scholar
  11. 11.
    M. Kozak, 1986, Point Mutations Define a Sequence Flanking the AUG Initiation Codon That Modulates Translation by Eukaryotic Ribosomes, Cell 44: 283.PubMedCrossRefGoogle Scholar
  12. 12.
    M.K. Dush, J.M. Sikola, S.A. Khan, J.A. Tischfield, and P.J. Stambrook, 1985, Nucleotide Sequence and Organization of the Mouse Adenine Phosphoribosyltransferase Gene, Proc. Natl. Acad. Sci. USA 82: 2731.PubMedCrossRefGoogle Scholar
  13. 13.
    J.L. Yates, N. Warren, and B. Sugden, 1985, Stable Replication of Plasmids Derived from Epstein-Barr Virus in Various Mammalian Cells, Nature 313: 812.PubMedCrossRefGoogle Scholar
  14. 14.
    R.B. DuBridge, P. Tang, H.C. Hsia, P-M. Leong, J.H. Miller, and P. Calos, 1987, Analysis of Mutation in Human Cells by Using an Epstein-Barr Virus Shuttle System, Mol. Cell. Biol. 7: 379.PubMedGoogle Scholar
  15. 15.
    M. Kozak, 1983, Comparison of Initiation of Protein Synthesis in Procaryotes, Eucaryotes, and Organelles, Microbiol. Rev. 47: 1.PubMedGoogle Scholar
  16. 16.
    M. Brombach and C.L. Pon, 1987, The Unusual Translation Initiation Codon AUU Limits the Expression of the infC (Initiation Factor IF3) Gene of Escherichia coli, Mol. Gen. Genet. 208: 94.PubMedCrossRefGoogle Scholar
  17. 17.
    F. Sherman, G. McKnight, and J.W. Stewart, 1980, AUG is the Only Initiation Codon in Eukaryotes, Biochim. Biophys. Acta 609: 343.PubMedGoogle Scholar
  18. 18.
    M. Kozak, 1981, Possible Role of Flanking Nucleotides in Recognition of the AUG Initiation Codon by Eukaryotic Ribosones, Nucleic Acids Res. 9: 5233.PubMedCrossRefGoogle Scholar
  19. 19.
    J.C. Brown and A.E. Smith, 1970, Initiation Codons in Eukaryotes, Nature 226: 610.PubMedCrossRefGoogle Scholar
  20. 20.
    C.W. Anderson and E. Buzash-Pollert, 1983, Can ACG Serve as an Initiation Codon for Protein Synthesis in Eucaryotic Cells?, Mol. Cell. Biol. 5: 3621.Google Scholar
  21. 21.
    O. Olsen, 1987, Yeast Cells May Use AUC or AAG as Initiation Codon for Protein Synthesis, Carlsberg Res. Commun. 52: 83.CrossRefGoogle Scholar
  22. 22.
    R.S. Zitomer, D.A. Walthall, B.C. Raymond, and C.P. Hollenberg, 1984, Saccharomyces cerevisiae Ribosomes Recognize Non-AUG Initiation Codons, Mol. Cell. Biol. 4: 1191.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Howard V. Hershey
    • 1
  • Milton W. Taylor
    • 1
  1. 1.Department of BiologyIndiana UniversityBloomingtonUSA

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