Sensitivity to Purine Analogues in Childhood Leukemia Assessed by the Automated MTT-Assay

  • R. Pieters
  • D. R. Huismans
  • A. Leyva
  • A. J. P. Veerman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)

Abstract

In the last two decades the survival rate of children with acute lymphoblastic leukemia (ALL) has improved greatly. However, with a complete remission percentage close to 100%, about 35% of the children still get a relapse. In a previous study it was shown that in childhood common-ALL, 5′nucleotidase (5′NT) positive cases have a poorer prognosis than 5′NT negative cases (Veerman et al., 1985). This might be due to the breakdown of the cytotoxic nucleotides of 6-MP by high cytoplasmic 5′NT activities. Alternatively, ecto 5′NT can provide purine requirements of the purine salvage pathway and therefore rescue cells from a blockage of purine de novo synthesis by 6-MP and/or MTX (Thompson, 1985; Pieters et al., 1987a). Thirdly, 5′NT positive cases might be relatively resistant to cytostatic drugs in general.

Keywords

Leukemia Sarcoma Methotrexate Glucocorticoid Pyrimidine 

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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • R. Pieters
    • 1
  • D. R. Huismans
    • 1
  • A. Leyva
    • 2
  • A. J. P. Veerman
    • 1
  1. 1.Department of PediatricsFree University HospitalAmsterdamThe Netherlands
  2. 2.Department of OncologyFree University HospitalAmsterdamThe Netherlands

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