Purine and Pyrimidine Metabolism of Normal and Leukemic Lymphocytes

  • Yolanda M. T. Marijnen
  • Dirk de Korte
  • Dirk Roos
  • Albert H. van Gennip
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)


Abnormalities in purine and pyrimidine metabolism may be associated with cell-cycle phase, proliferation, immaturity or transformation. Therefore, detailed information concerning purine and pyrimidine pattern in malignant cells compared to normal cells is needed. Abnormal enzyme activities (1) as well as abnormal metabolite concentrations (2) have been described for leukemic cells.


Adenosine Deaminase Pyrimidine Metabolism Pyrimidine Synthesis Adenosine Deaminase Activity Leukemic Lymphocyte 
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  1. 1).
    Drexler, H.G., Gaedicke, G. and Minowada, J., Enzyme markers in acute leukemia: Advances during the laste decade, J. Natl. Cancer Inst, 72, 1283–1298 (1984).PubMedGoogle Scholar
  2. 2).
    De Korte, D., Haverkort, W.A., Roos, D, Behrendt, H and Van Gennip, A.H., Imbalance in the ribonucleotide pools of lymphoid ceils from acute lymphoblastic leukemia patients, Leukemia Res., vol.10, 389–396 (1986).CrossRefGoogle Scholar
  3. 3).
    De Korte, D., Haverkort, W.A., Van Gennip, A.H., Roos, D., Nucleotide profiles of normal human blood cells determined by high-performance liquid chromatography, Analytical Biochem., 147, 197–209 (1985).CrossRefGoogle Scholar
  4. 4).
    Van Lier, R.A.W., Boot, J.K.A., De Groot, E.R. and Aarden, L.A., Induction of T cell proliferation with anti-CD3 switch variant monoclonal antibodies: effects of heavy chain isotype in monocyte-dependent systems, Eur. J. Immunol., 17, 1599–1604 (1987).PubMedCrossRefGoogle Scholar
  5. 5).
    Sauerwein, R.W., Van der Meer, W.G.J. and Aarden, L.A., Analysis of T-cell replacing factor-like activity: potent induction of T helper activity for human B cells by residual concanavalin A and interleukin 2, Eur. J. Immunol., 17, 1145–1150 (1987).PubMedCrossRefGoogle Scholar
  6. 6).
    De Korte, D., Marijnen, Y.M.T., Haverkort, W.A., Van Gennip, A.H. and Roos, D., Sensitive on-line radioactivity measurements with a heterogeneous flow-cell: application to lymphoid cell ribonucleotides separated by high-performance liquid chroma-tography, J. Chromatography, 415, 383–387 (1987).CrossRefGoogle Scholar
  7. 7).
    Jackson, R.C. and Weber, G., IMP dehydrogenase, an enzyme linked with proliferation and malignancy, Nature, 256, 331–333 (1975).PubMedCrossRefGoogle Scholar
  8. 8).
    Simpkins, H., Stanton, A. and Davis, B.H., Adenosine deaminase activity in lymphoid subpopulations and leukemias. Cancer Res., 41, 3107–3110 (1981).PubMedGoogle Scholar
  9. 9).
    Raivio, K.O. and Hovi, T., Purine re-utilization in phytohemagglutinin-stimulated human T lymphocytes. Exp. Cell Res. 116, 75–83.Google Scholar
  10. 10).
    Ellims, P.H., Gan, T.E. and Medley, G., Cytidine triphosphate synthetase activity in lymphoproliferative disorders, Cancer Res., 43, 1432–1435 (1983).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Yolanda M. T. Marijnen
    • 1
  • Dirk de Korte
    • 1
  • Dirk Roos
    • 1
  • Albert H. van Gennip
    • 1
    • 2
  1. 1.Central Lab.Netherl.Red Cross Blood Transf. Service, incorp. Lab. of Exp. and Clin. Immunol.Univ. of AmsterdamThe Netherlands
  2. 2.Children’s Hosp. “Het Emma Kinderzie-kenhuis”AmsterdamThe Netherlands

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