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Degradation of Purine Nucleosides by Mitochondrial Enzymes of Bovine Liver

  • Roger A. Lewis
  • Laura Link
  • Wilbert Chen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)

Abstract

In recent years, it was shown that mitochondria actively metabolize purine deoxynucleosides. It was documented that both deoxyguanosine and deoxyadenosine were phosphorylated by mitochondrial enzymes (1–6). In the case of deoxyguanosine, intact mitochondria formed dGMP, dGDP and dGTP (3, 5). dAMP was detected in intact mitochondria which were supplemented with deoxyadenosine (6). Regarding the degradation of purine deoxynucleosides by mitochondrial enzymes, adenosine deaminase activity was noted in intact and fractured mitochondria (6, 7).

Keywords

Adenosine Deaminase Mitochondrial Enzyme Purine Nucleoside Purine Nucleoside Phosphorylase Ammonium Sulfate Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    W.R. Gower, M.C. Carr, and D.H. Ives, Deoxyguanosine Kinase, J. Biol. Chem. 254: 2180 (1979).PubMedGoogle Scholar
  2. 2.
    K. Fabianowska-Majewska, J. Greger, and B. Gorzkiewicz, Purification and Properties of Deoxyadenosine Kinase from Rat Liver Mitochondria, Enzyme 31: 27 (1984).PubMedGoogle Scholar
  3. 3.
    R.A. Lewis and L.F. Watkins, Phosphoryation of Deoxyguanosine in Rat Liver Mitochondria, in “Purine Metabolism in Man-IV, Part B,” C.H.M.M. De Bruyn, H.A. Simmonds, and M.M. Muller, eds., Plenum Press, New York (1984).Google Scholar
  4. 4.
    L.F. Watkins and R.A. Lewis, The Metabolism of Deoxyguanosine in Mitochondria: A Characterization of the Phosphorylation Process Which Occurs in Intact Mitochondria, Biochim. Biophys. Acta 923: 103 (1987).PubMedCrossRefGoogle Scholar
  5. 5.
    L.F. Watkins and R.A. Lewis, Phosphorylation of Deoxyguanosine in Intact and Fractured Mitochondria, Molec. Cell Biochem. 77: 153 (1987).PubMedGoogle Scholar
  6. 6.
    R.G. Seals and R.A. Lewis, Deoxyadenosine Deamination and Phosphorylation in Rat Liver Mitochondria, Comp. Biochem. Physiol. 88B: 939 (1987).Google Scholar
  7. 7.
    J. Greger and K. Fabianowska, Relationship Between 5′-Nucleotidase, Adenosine Deaminase, AMP Deaminase, ATP(Mg2+)-ase Activities and dTMP Kinase Activity in Rat Liver Mitochondria, Enzyme 24: 54 (1979).PubMedGoogle Scholar
  8. 8.
    J.B. Chappell and R.G. Hansford, Preparation of Mitochondria from Animal Tissues and Yeasts, in “Subcellular Components. Preparation and Fractionation,” G.B. Bernie, ed., Butterworth, London (1972).Google Scholar
  9. 9.
    R.P. Agarwal and R.E. Parks, Jr., Adenosine Deaminase from Human Erythrocytes in “Methods in Enzymology, LI,” P.A. Hoffee and M.E. Jones, eds., Academic Press, New York (1978).Google Scholar
  10. 10.
    P.A. Hoffee, R. May, and B.C. Robertson, Purine Nucleoside Phosphorylase from Salmonella Typhimurium and Rat Liver, in “Methods in Enzymology, LI,” P.A. Hoffee and M.E. Jones, eds., Academic Press, New York (1978).Google Scholar
  11. 11.
    S. Cha, R.P. Agarwal and R.E. Parks, Jr., Tight-Binding Inhibitors II. Non-steady State Nature of Inhibition of Milk Xanthine Oxidase by Allopurinol and Alloxanthine and of Human Erythrocyte Adenosine Deaminase by Coformycin, Biochem. Pharmacol. 24: 2187 (1975).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Roger A. Lewis
    • 1
  • Laura Link
    • 1
  • Wilbert Chen
    • 1
  1. 1.Department of BiochemistryUniversity of Nevada-RenoRenoUSA

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