Advertisement

Urate Binding Globulin, Interactions with Immunoglobulins

  • Maria R. Mazzoni
  • Gino Giannaccini
  • Fabio Lena
  • Claudia Martini
  • Laura Bazzichi
  • Maria L. Ciompi
  • Antonio Lucacchini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 253A)

Abstract

The presence of an urate binding to serum proteins may influence the deposition in joints, and the renal excretion of urate. So several authors have studied these interactions with different results. By use of an affinity chromatography procedure we demonstrated the presence of at least two different serum proteins which recognize urate: albumin and α-globulin (1). Application of this technique led to isolation and purification of the urate binding globulin from human serum (2). This protein, which migrates as α2 globulin is devoid of enzymatic activity, shows an apparent molecular weight of 70000 + 4000 Daltons and it has been partially characterized as previously reported (3). In this paper we describe the presence of an urate binding globulin-IgG complex in human plasma or plasma fraction III according to Cohn.

Keywords

Uric Acid Serum Protein Ammonium Sulphate Affinity Column Apparent Molecular Weight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M.L. Ciompi, A. Lucacchini, D. Segnini, M.R. Mazzoni, Urate-binding proteins in plasma studied by affinity chromatography, in “Purine Metabolism in Man-Ill”, A. Rapado, R.W.E. Watts, C.H.M.M. De Bruyn eds., Plenum Press, New York (1980)Google Scholar
  2. 2.
    M.R. Mazzoni, C. Martini, D. Segnini, M.L. Ciompi, A. Lucacchini, Isolation of an urate-binding proteinby affinity chromatography, Appl. Biochem. Biotech. 7: 189 (1982)CrossRefGoogle Scholar
  3. 3.
    M.R. Mazzoni, M.L. Ciompi, G. Pasero, C. Martini, D. Segnini, A. Lucacchini, Characterzation of an urate binding 2-globulin from human serum, in “Purine Metabolism in Man-IV”, C.H.M.M. De Bruyn, H.A. Simmonds, M.M. Muller eds., Plenum Press, New York (1984)Google Scholar
  4. 4.
    B.J. Davis, Disc electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121: 404 (1964)PubMedCrossRefGoogle Scholar
  5. 5.
    B.D. Hames, An introduction to polyacrylamide gel electrophoresis, in “Gel electrophoresis of proteins”, B.D. Hames, D. Rickwood eds., IRL Press, Oxford (1985)Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Maria R. Mazzoni
    • 1
  • Gino Giannaccini
    • 1
  • Fabio Lena
    • 1
  • Claudia Martini
    • 1
  • Laura Bazzichi
    • 2
  • Maria L. Ciompi
    • 2
  • Antonio Lucacchini
    • 1
  1. 1.Istituto Policattedra di Discipline, BiologicheUniversity of PisaItaly
  2. 2.Istituto Patologia Speciale MedicaUniversity of PisaItaly

Personalised recommendations