Mycotoxins in North Carolina 1985 Crop Soybeans. I. Zearalenone and Deoxynivalenol in Soybeans and Soybean Meal

  • Winston M. HaglerJr.
  • Frank T. Jones
  • Daryl T. Bowman


Soybean meal is the most important source of protein in animal feeds in the United States. Soybeans (Glycine max L.) and soybean meal have been perceived to be poor substrates for fungal growth. They have enjoyed the reputation of being resistant to contamination with mycotoxins, particularly aflatoxins and zearalenone (Shotwell et al., 1969; Eugenio et al., 1970), in comparison to corn, wheat, sorghum, and other feed grains. Thus, commercial soybean products have been thought to be very low in or free from fungal metabolites.


Soybean Meal Proximate Analysis Mycotoxin Contamination Soybean Sample Crop Bean 
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  1. Allen, R.D. (1986). Ingredient analysis table: 1987 edition. Feedstuffs, 59, 25–30.Google Scholar
  2. Anon. (1972). Atlas of Nutritional Data on United States and Canadian Feeds. p. 630, Nat. Acad. Sci., Washington, DC, 772 pp.Google Scholar
  3. Anon. (1969). Official and Tentative Methods. ( 3rd ed. ), Amer. Oil Chem. Soc., Champaign, IL.Google Scholar
  4. Babadoost, M., Hagler, W.M., Jr., Bowman, D.T., and Nelson, P.E. (1987). Field contamination of sorghum with zearalenone and deoxynivalenol in North Carolina: Density segregation to remove mycotoxins. In: Biodeterioration Research I. pp. 99–109 ( G.C. Llewellyn and C.E. O’Rear, eds.), Plenum Publishing Co., New York.Google Scholar
  5. Barr, A.J., Goodnight, J.H., Sall, J.P., and Helwig, J.T. (1976). A User’s Guide to SAS 76. SAS Inst., Inc., Raleigh, NC.Google Scholar
  6. Bean, G.A., Schillinger, J.A., and Klarman, W.L. (1972). Occurrence of aflatoxin and aflatoxin-producing strains of Aspergillus spp. in soybeans. Appl. Microbiol., 24, 437–439.Google Scholar
  7. Emeh, C.O., and Marth, E.H. (1976). Rubratoxin formation in soybean substrates. J. Milk Food Technol., 39, 253–257.Google Scholar
  8. Eugenio, C.P., Christensen, C.M., and Mirocha, C.J. (1970). Factors affecting production of mycotoxin F-2 by Fusarium roseum. Phytopathology, 60, 1055–1057.CrossRefGoogle Scholar
  9. Gupta, S.K., and Venkitasubramanian, T.A. (1975). Production of aflatoxin on soybeans. Appl. Microbiol., 29, 834–836.Google Scholar
  10. Hamilton, R.M.G., and Trenholm, H.L. (1984). Observations on the chemical and nutrient content of white winter and spring wheats contaminated with deoxynivalenol (vomitoxin). Anim. Feed Sci. Technol., 11, 293–300.Google Scholar
  11. Hutchins, J.E., and Hagler, W.M., Jr. (1983a). Rapid determination of aflatoxins in heavily contaminated corn. J. Assoc. Offic. Anal. Chem., 64, 1458–1465.Google Scholar
  12. Hutchins, J.E., and Hagler, W.M., Jr. (1983b). Derivatization of aflatoxins B1 and G1 for high performance liquid chromatograhic determination. Assoc. Offic. Anal. Chem., Abstr., 179, 72.Google Scholar
  13. Jones, F.T., Hagler, W.M., Jr., and Hamilton, P.B. (1982). Association of low levels of aflatoxin in feed with productivity losses in commercial broiler operations. Poultry Sci., 61, 861–868.Google Scholar
  14. Jordan, E.G., Manandhur, J.B., Thapliyal, P.N., and Sinclair, J.B. (1986). Factors affecting soybean seed quality in Illinois. Plant Dis., 70, 246–248.CrossRefGoogle Scholar
  15. Kamimura, H., Nishijima, M., Yasuda, K., Saito, K., Ibe, A., Nagayama, T., Ushiyama, H., and Naoi, Y. (1981). Simultaneous detection of several Fusarium mycotoxins in cereals, grains, and foodstuffs. J. Assoc. Offic. Anal. Chem., 64, 1067–1073.Google Scholar
  16. Lisker, N., Ben-Efraim, A., and Henis, Y. (1985a). Fungi growing on stored soybeans and their significance in lipid breakdown. Ann. Appl. Biol., 107, 117–126.Google Scholar
  17. Lisker, N., and Henis, Y. (1985b). Involvement of fungi in the increase of free fatty acids in stored soybeans. Can. J. Microbiol., 31, 799–803.Google Scholar
  18. Nash, S.M., and Snyder, W.C. (1962). Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology, 52, 567–572.Google Scholar
  19. Richardson, K.E., Hagler, W.M., Jr., Haney, C.A., and Hamilton, P.B. (1985). Zearalenone and trichothecene production in soybeans by toxigenic Fusarium. J. Food Protect., 48, 240–243.Google Scholar
  20. Romer, T.R. (1986). Use of small charcoal alumina cleanup columns in determination of trichothecene mycotoxins in foods and feeds. J. Assoc. Offic. Anal. Chem., 69, 699–703.Google Scholar
  21. Saio, K., Nikkuni, I., Ando, Y., Otsuru, M., Terauchi, Y., and Kito, M. (1980). Soybean quality changes during model storage studies. Cereal Chem., 57, 77–82.Google Scholar
  22. Shotwell, O.L., Hesseltine, C.W., Burmeister, H.R., Kwolek, W.F., Shannon, G.M., and Hall, H.H. (1969). Survey of cereal grains and soybeans for the presence of aflatoxin. II. Corn and soybeans. Cereal Chem., 46, 454–463.Google Scholar
  23. Swanson, S.P., Corley, R.A., White, D.G., and Buck, W.B. (1984). Rapid thin layer chromatographic method for the determination of zearalenone and zearalenol in grains and animal feeds. J. Assoc. Offic. Anal. Chem., 67, 580–582.Google Scholar
  24. Trenholm, H., Warner, R.M., and Prelusky, D.M. (1985). Assessment of extraction procedures in the analysis of naturally contaminated grain products for deoxynivalenol (vomitoxin). J. Assoc. Offic. Anal. Chem., 68, 645–649.Google Scholar
  25. Tyczkowska, K., Hutchins, J.E., and Hagler, W.M., Jr. (1984). Determination of ochratoxin A in feeds and biological fluids using prepacked celite columns. Assoc. Offic. Anal. Chem., Abstr., 134, 13.Google Scholar
  26. Wicklow, D.G., Bennett, G.A., and Shotwell, O.L. (1987). Secondary invasion of soybeans by Fusarium Qraminearum and resulting mycotoxin contamination. Plant Dis., 71, 1146.CrossRefGoogle Scholar
  27. Wilcox, J.R., Laviolette, F.A., and Athow, K.L. (1974). Deterioration of soybean seed quality associated with delayed harvest. Plant Dis. Reptr., 58, 130–133.Google Scholar
  28. Williams, S. (1984). Official Methods of Analysis. ( 13th ed. ), Assoc. Offic. Anal. Chem., Arlington, VA., 1141 pp.Google Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Winston M. HaglerJr.
    • 1
  • Frank T. Jones
    • 2
  • Daryl T. Bowman
    • 3
  1. 1.Mycotoxin Laboratory, Department of Poultry ScienceN.C. State UniversityRaleighUSA
  2. 2.Department of Poultry ScienceN.C. State UniversityRaleighUSA
  3. 3.Department of Crop ScienceN.C. State UniversityRaleighUSA

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