Abstract
Disintegration of animal or plant tissue results in a complex mixture of intact organelles and membrane vesicles. To obtain a homogeneous preparation of a certain organelle or membrane is, therefore, usually a very demanding task, particularly since the organelles we want should not only be morphologically intact but also fully active, and the membrane vesicles should not only be homogeneous with respect to origin (including domains) but also with respect to orientation (sidedness), and, in addition, retain their activities. To fulfil these demands we can usually not rely on one method only but have to combine different methods separating according to different properties of the particles. This makes two-phase partitioning an extremely attractive method since it separates particles according to differences in surface properties [1], and therefore complements differential and gradient centrifugation which separate according to size and density.
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References
P.-Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd edition, John Wiley & Sons, New York (1986)
S.D. Flanagan, Partitioning of animal membranes and organelles, in: “Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses and Applications to Biotechnology,” H. Walter, D.E. Brooks and D. Fisher, eds., Academic Press, Orlando, FA (1985)
A. Mircheff, Empirical strategy for analytical fractionation of epithelial cells, Am. J. Phys. 244:G347 (1983)
P.-Å. Albertsson, B. Andersson, C. Larsson and H.-E. Åkerlund, Phase partition — a method for purification and analysis of cell organelles and membrane vesicles, in: “Methods of Biochemical Analysis,” D. Glick, ed., John Wiley & Sons, New York (1982)
C. Larsson, Partition in aqueous polymer two-phase systems: A rapid method for separation of membrane particles according to their surface properties, in: “Isolation of Membranes and Organelles from Plant Cells,” Academic Press, London (1983)
C. Larsson, B. Andersson and H.-E. Åkerlund, Partitioning of plant cells, cell walls, membranes and organelles, in: “Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses and Applications to Biotechnology,” H. Walter, D.E. Brooks and D. Fisher, eds., Academic Press, Orland, FA (1985)
C. Larsson, S. Widell and P. Kjellbom, Preparation of high-purity plasma membranes, Methods Enzymol. 148:558 (1987)
P. Gardeström, I. Ericson and C. Larsson, Preparation of mitochondria from green leaves of spinach by differential centrifugation and phase partition, Plant Sci. Lett. 13:231 (1978)
T.L. Steck, Preparation of impermeable inside-out and right-side-out vesicles from erythrocyte membranes, in: “Methods in Membrane Biology,” Plenum Press, New York (1974)
J.M. Anderson and B. Andersson, The architecture of photosynthetic membranes: Lateral and transverse organisation, Trends Biochem. Sci. 7:288 (1982)
I.M. Moller, A. Bergman, P. Gardeström, I. Ericson and J.M. Palmer, Characterisation and purification of inside-out submitochondrial particles obtained from plant mitochondria, FEBS Lett. 126:13 (1981)
C. Larsson, S. Widell and M. Sommarin, Inside-out plant plasma membrane vesicles of high purity obtained by aqueous two-phase partitioning, FEBS Lett. 229:289 (1988)
H.-E. Åkerlund, An apparatus for countercurrent distribution in a centrifugal acceleration field, J. Biochem. Biophys. Methods 9:133 (1984)
P. Gierow, B. Jergil and P.-Å. Albertsson, Two-phase partitioning of rat liver plasma membranes, in: “Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology,” D. Fisher and I.A. Sutherland, eds., Plenum, New York (1989)
H. Canut, A. Brightman, A. Boudet and D.J. Morré, Free-flow electrophoresis resolves plasma membrane vesicles isolated by aqueous two-phase partition into two populations of different sidedness, in: “Separations Using Aqueous Phase Systems: Applications in Cell Biology and Biotechnology,” D. Fisher and I.A. Sutherland, eds., Plenum, New York (1989)
P. Gierow, B. Jergil and P.-Å. Albertsson, Lateral heterogeneity of rat liver plasma membranes analysed by countercurrent distribution, Biochem. J. 249:369 (1988)
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© 1989 Plenum Press, New York
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Larsson, C. (1989). Separation of Organelles and Membranes by Phase Partitioning — A Short Overview. In: Fisher, D., Sutherland, I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5667-7_9
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DOI: https://doi.org/10.1007/978-1-4684-5667-7_9
Publisher Name: Springer, Boston, MA
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